对感染疟原虫的人类红细胞进行分级分离以研究蛋白质运输。
Fractionation of Plasmodium-infected human red blood cells to study protein trafficking.
作者信息
Külzer Simone, Bittl Verena, Przyborski Jude M
机构信息
Research School of Biology, The Australian National University, Canberra, Australia.
出版信息
Methods Mol Biol. 2015;1270:71-80. doi: 10.1007/978-1-4939-2309-0_5.
Subcellular fractionation is a valuable tool to follow protein traffic between cellular compartments. Here we detail a procedure for fractionating erythrocytes infected with the human malaria parasite P. falciparum using the bacterial pore-forming protein Streptolysin O (SLO). Additionally we describe an experimental protocol to determine protein topology by carrying out a protease protection assay on SLO-lysed infected erythrocytes.
亚细胞分级分离是追踪细胞区室间蛋白质运输的一种有价值的工具。在此，我们详细介绍一种使用细菌成孔蛋白链球菌溶血素O（SLO）对感染人类疟原虫恶性疟原虫的红细胞进行分级分离的方法。此外，我们还描述了一种实验方案，通过对经SLO裂解的感染红细胞进行蛋白酶保护试验来确定蛋白质拓扑结构。
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