Appino S, Vincenti L, Rota A, Pellegrini S, Chieppa M N, Cadoni V, Pregel P
Dipartimento di Medicina Veterinaria, University of Sassari, Sassari, Italy.
Reprod Domest Anim. 2015 Jun;50(3):526-8. doi: 10.1111/rda.12505. Epub 2015 Feb 21.
Fifty-seven genital tracts of regularly slaughtered culled Piedmontese cows, aged 7.4 ± 4.3 years (mean ± SD), range: 2.6-15.6 years, were grossly and microscopically examined. DNA extracted from oviducts was subjected to PCR to evaluate the presence of Chlamydia spp. The 15 PCR-positive oviducts were subjected to Sanger sequencing and showed the presence of Chamydia abortus, with an identity range between 99 and 100%. Nine of the PCR-positive samples belonged to the 24 animals with a normal macroscopic appearance of the whole genital tract (percentage of positive oviducts in normal genital tracts 9/24 = 37.5%), while six belonged to the 33 genital tracts with lesions in one or more organs (percentage of positive oviducts in pathological genital tracts 6/33 = 18.1%); of these, a single animal had salpingitis. The detection of C. abortus in bovine oviducts is of particular interest because it has never been previously investigated or reported.
对57头定期屠宰淘汰的皮埃蒙特奶牛的生殖道进行了大体和显微镜检查，这些奶牛年龄为7.4±4.3岁（平均值±标准差），范围为2.6 - 15.6岁。从输卵管提取的DNA进行PCR检测以评估衣原体属的存在情况。对15个PCR阳性的输卵管进行桑格测序，结果显示存在流产衣原体，同一性范围在99%至100%之间。9个PCR阳性样本属于24头整个生殖道外观正常的动物（正常生殖道中阳性输卵管的百分比为9/24 = 37.5%），而6个属于33个一个或多个器官有病变的生殖道（病理性生殖道中阳性输卵管的百分比为6/33 = 18.1%）；其中，仅有1头动物患有输卵管炎。在牛输卵管中检测到流产衣原体特别令人关注，因为此前从未对其进行过研究或报道。
