Borrelli G M, Kaufmann A M, Abrão M S, Mechsner S
Charité Universitätsmedizin Berlin - Gynecology Clinic, Campus Benjamin Franklin, Berlin, Germany; University of Sao Paulo, Faculty of Medicine - Department of Obstetrics and Gynecology, Brazil.
Charité Universitätsmedizin Berlin - Gynecology Clinic, Campus Benjamin Franklin, Berlin, Germany.
J Reprod Immunol. 2015 Jun;109:66-73. doi: 10.1016/j.jri.2015.01.003. Epub 2015 Feb 7.
Chemokines have been associated with endometriosis. Our study was aimed at evaluating the levels of six chemokines--CXCL8 (IL-8), CXCL12 (SDF-1), CCL2 (MCP-1), CCL5 (RANTES), CCL19 (MIP-3β), and CCL21 (6-Ckine)--in the peritoneal fluid (PF) of patients with and controls without endometriosis by multiplexed cytokine assay. In this retrospective case-control study conducted at the Charité University Hospital, patients (n = 36) and controls (n = 27) were enrolled. The patients were separated into groups according to stage of the disease: I-II (n = 21), III-IV (n = 1 5), and according to clinical findings: peritoneal endometriosis (PE; n = 7), deep-infiltrating endometriosis (DIE) affecting the retrocervical area (n = 13) or the bowel/rectovaginal site (n = 14). The subjects were also separated according to the cycle phase: follicular (n = 14) or luteal (n = 8) and the previous use (n = 25) or not (n = 38) of hormones. PF was collected from all subjects (n = 63) consecutively during laparoscopy. The concentration of chemokines in the PF was assessed using Luminex(®) x-MAP(®) technology. Sensitivity and specificity were calculated. A model of multiple logistic regressions estimated the odds of endometriosis for each combination of the chemokines detected. We observed significantly higher concentrations of IL-8 (p < 0.001), MCP-1 (p = 0.014), and MIP-3β (p = 0.022) in the PF of women with endometriosis than in the controls. A joint evaluation revealed that elevated levels of the three chemokines had a positive endometriosis prediction value of 89.1%. The combined assessment of MCP-1, MIP-3β, and IL-8 concentration in PF improved the likelihood of identifying patients with endometriosis. Future studies should investigate this panel in peripheral blood samples.
趋化因子与子宫内膜异位症有关。我们的研究旨在通过多重细胞因子检测法评估患有子宫内膜异位症的患者及未患该病的对照者腹腔液（PF）中六种趋化因子——CXCL8（白细胞介素-8）、CXCL12（基质细胞衍生因子-1）、CCL2（单核细胞趋化蛋白-1）、CCL5（调节激活正常T细胞表达和分泌因子）、CCL19（巨噬细胞炎性蛋白-3β）和CCL21（6-Ckine）——的水平。在夏里特大学医院进行的这项回顾性病例对照研究中，纳入了患者（n = 36）和对照者（n = 27）。患者根据疾病分期分为：I-II期（n = 21）、III-IV期（n = 15），并根据临床表现分为：腹膜子宫内膜异位症（PE；n = 7）、累及宫颈后区域（n = 13）或肠道/直肠阴道部位（n = 14）的深部浸润性子宫内膜异位症（DIE）。受试者还根据月经周期阶段分为：卵泡期（n = 14）或黄体期（n = 8），以及是否曾使用过激素（n = 25）或未使用过激素（n = 38）。在腹腔镜检查期间连续收集所有受试者（n = 63）的PF。使用Luminex(®) x-MAP(®)技术评估PF中趋化因子的浓度。计算敏感性和特异性。多元逻辑回归模型估计了检测到的每种趋化因子组合的子宫内膜异位症发生几率。我们观察到，患有子宫内膜异位症的女性PF中白细胞介素-8（p < 0.001）、单核细胞趋化蛋白-1（p = 0.014）和巨噬细胞炎性蛋白-3β（p = 0.022）的浓度显著高于对照组。联合评估显示，这三种趋化因子水平升高对子宫内膜异位症的预测阳性值为89.1%。对PF中单核细胞趋化蛋白-1、巨噬细胞炎性蛋白-3β和白细胞介素-8浓度的综合评估提高了识别子宫内膜异位症患者的可能性。未来的研究应在外周血样本中研究该指标组合。
