苏丹人群鼻咽癌中爱泼斯坦-巴尔病毒的分子检测
Molecular Detection of Epstein - Barr virus in Nasopharyngeal Carcinoma among Sudanese population.
作者信息
Edreis Ali, Mohamed Mona Ali, Mohamed Nouh S, Siddig Emmanuel E
机构信息
Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.
Department of Parasitology and Medical Entomology, Faculty of Medical Laboratory Sciences, Nile College, Khartoum, Sudan.
出版信息
Infect Agent Cancer. 2016 Nov 8;11:55. doi: 10.1186/s13027-016-0104-7. eCollection 2016.
BACKGROUND
Nasopharyngeal carcinoma (NPC) is the most common cancer arising from the nasopharynx that varies significantly from other cancers of the head and neck in its occurrence, causes, clinical behavior, and treatment. NPC caused by an interaction between infection with EBV and environmental and genetic factors, encompasses a multistep oncogenic process. The frequency of Epstein-Barr virus EBV among nasopharyngeal carcinoma is well known worldwide, however, in the Sudan there is barely a published data. The aim of this study was to detect Epstein-Barr virus (EBV) in nasopharyngeal carcinoma (NPC) biopsies obtained from Sudanese patients using Polymerase Chain reaction.
METHODS
This is a descriptive, retrospective hospital based study, conducted at the National Center for ENT diseases and the Faculty of Medical Laboratory Science, University of Khartoum, Khartoum City, Sudan. Archival blocks were obtained from 82 patients diagnosed as having nasopharyngeal carcinoma were molecularly examined for the presence of Epstein-Barr virus.
RESULTS
Eighty two Paraffin fixed tissue sections were examined for the presence of the virus using PCR, EBV was identified in 51/ 82 (62.2 %) samples and couldn't be identified in 31/ 82 (37.8 %) tissue samples. Out of the 51 infected samples, 33/51 (64.7 %) were found among males and 18/27 (66.7 %) were found among females.
CONCLUSION
The present study is providing strong evidence supporting the general association of EBV infection in NPC among Sudanese patients.
背景
鼻咽癌（NPC）是鼻咽部最常见的癌症，其在发病率、病因、临床行为和治疗方面与其他头颈癌有显著差异。鼻咽癌由EB病毒感染与环境和遗传因素相互作用引起，涉及一个多步骤的致癌过程。EB病毒（EBV）在鼻咽癌中的感染率在全球范围内广为人知，然而，在苏丹几乎没有公开的数据。本研究的目的是使用聚合酶链反应检测从苏丹患者获得的鼻咽癌（NPC）活检组织中的EB病毒（EBV）。
方法
这是一项基于医院的描述性回顾性研究，在苏丹喀土穆市喀土穆大学医学检验科学学院和国家耳鼻喉疾病中心进行。从82例被诊断为鼻咽癌的患者获取存档组织块，对其进行EB病毒存在情况的分子检测。
结果
使用PCR对82个石蜡固定组织切片进行病毒检测，在51/82（62.2%）的样本中鉴定出EBV，在31/82（37.8%）的组织样本中未检测到。在51个感染样本中，男性中有33/51（64.7%），女性中有18/27（66.7%）。
结论
本研究提供了有力证据，支持苏丹患者鼻咽癌中EBV感染的普遍关联。
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