Manolescu Bogdan Nicolae, Cinteza Delia, Berteanu Mihai, Busu Carmina, Oprea Eliza
"C.D. Nenitescu" Department of Organic Chemistry, Faculty of Applied Chemistry and Science of Materials, Polytechnic University of Bucharest, Bucharest, Romania.
Department of Rehabilitation and Physical Medicine, Faculty of Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
Maedica (Bucur). 2014 Sep;9(3):248-54.
Stroke is a pathological condition associated with a redox imbalance. Both the acute and the post-acute phases after a stroke are characterized by a pro-oxidant state, which could be corrected through antioxidant supplementation. The aim of the present study was to evaluate the effect of the ALAnerv(®) upon the redox status of erythrocytes.
For this pilot study were enrolled 28 post-acute stroke patients. They were randomly divided into a control group [(-) ALA] and a study group [(+) ALA]. Patients were hospitalized for a period of two weeks. Blood samples were taken at the beginning and at the end of this period. Patients from (+) ALA group received the nutritional supplement ALAnerv(®). Catalase, SOD, GPx, GRed and GT activities were assessed on erythrocytes' lysates. Also, the total antioxidant capacity as well as the concentration of total thiols were evaluated.
During the study period only SOD (1393.48 ± 69.14 U/g Hb vs. 1514.06 ± 60.80) and GRed (4.47 ± 0.37 U/g Hb vs. 5.06 ± 0.24) significantly increased in (+) ALA group. Catalase (173.70 ± 14.62 k/g Hb vs. 123.41 ± 8.71) and GT (5.55 ± 0.26 U/g Hb vs. 4.95 ± 0.23) activities significantly decreased during the study period. Multiple regression indicated that only SOD, GT and GRed activities were influenced by the ALAnerv(®) consumption. There was no significant increase if the total thiols concentration.
ALAnerv(®) consumption could be associated with a correction of the erythrocytes' redox status. There is a need to investigate if administration of ALAnerv(®) over a longer time period could have a more significant influence on the erythrocytes' antioxidant system.
中风是一种与氧化还原失衡相关的病理状态。中风后的急性期和急性后期均以促氧化状态为特征，可通过补充抗氧化剂来纠正。本研究的目的是评估ALAnerv（®）对红细胞氧化还原状态的影响。
本初步研究纳入了28例急性后期中风患者。他们被随机分为对照组[（-）ALA]和研究组[（+）ALA]。患者住院两周。在此期间开始和结束时采集血样。（+）ALA组的患者接受营养补充剂ALAnerv（®）。对红细胞裂解物评估过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和谷胱甘肽转移酶活性。此外，还评估了总抗氧化能力以及总硫醇浓度。
在研究期间，仅（+）ALA组的超氧化物歧化酶（1393.48±69.14 U/g Hb对1514.06±60.80）和谷胱甘肽还原酶（4.47±0.37 U/g Hb对5.06±0.24）显著增加。研究期间过氧化氢酶（173.70±14.62 k/g Hb对123.41±8.71）和谷胱甘肽转移酶（5.55±0.26 U/g Hb对4.95±0.23）活性显著降低。多元回归表明，只有超氧化物歧化酶、谷胱甘肽转移酶和谷胱甘肽还原酶活性受ALAnerv（®）摄入的影响。总硫醇浓度没有显著增加。
服用ALAnerv（®）可能与红细胞氧化还原状态的纠正有关。有必要研究长时间服用ALAnerv（®）是否会对红细胞抗氧化系统产生更显著的影响。
