二嗪农对成年大鼠睾丸组织生化参数的单剂量效应及维生素E的保护作用
Single dose effect of diazinon on biochemical parameters in testis tissue of adult rats and the protective effect of vitamin E.
作者信息
Rahimi Anbarkeh Fatemeh, Nikravesh Mohammad Reza, Jalali Mehdi, Sadeghnia Hamid Reza, Sargazi Zinat, Mohammdzadeh Leila
机构信息
Department of Anatomy and cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Iran J Reprod Med. 2014 Nov;12(11):731-6.
BACKGROUND
Diazinon (DZN) is an organophosphate pesticide that widely used for agricultural pest control all over the world. DZN affects target organs including reproductive system by inhibiting the activity of acetylcholinesterase and inducing oxidative stress. Vitamin E (α-tocopherol) is a strong antioxidant which inhibits free radicals, and probably can reduce lipid perxidation effectively in biological systems.
OBJECTIVE
The present study, aimed to evaluate the effects of DZN on malondialdehyde (MDA) and glutathione (GSH) levels in testis of rats and protective effect of vitamin E.
MATERIALS AND METHODS
In this experimental study, thirty adult male Wistar rats (200-250 gr) were divided into 5 groups (n= 6): control group (did not receive any material), sham group (received only pure olive oil), experimental group 1 (DZN, 60 mg/kg), experimental group 2 (Vit E, 200 mg/kg) and experimental group 3 (DZN+Vit E, with the same dose). All groups were sacrificed after 6 weeks and right testis was used to measure the MDA and GSH levels. The amount of MDA was determined by the thiobarbituric acid assay and 5, 5-Dithio-bis (2nitrobenzoic acid) DTNB-recycling protocol was used for GSH assay.
RESULTS
The results showed that DZN increased MDA level (p<0.001) and reduced GSH level (p<0.001). Administration of DZN plus vitamin E decreased the MDA level (p<0.001) and increased GSH level (p=0.001).
CONCLUSION
DZN induced lipid peroxidation in the testis of rats. Vitamin E by its antioxidant activity was able to improve the toxic effect of DZN.
背景
二嗪农（DZN）是一种有机磷农药，在全球广泛用于农业害虫防治。DZN通过抑制乙酰胆碱酯酶的活性和诱导氧化应激来影响包括生殖系统在内的靶器官。维生素E（α-生育酚）是一种强大的抗氧化剂，可抑制自由基，并且可能在生物系统中有效降低脂质过氧化。
目的
本研究旨在评估DZN对大鼠睾丸中丙二醛（MDA）和谷胱甘肽（GSH）水平的影响以及维生素E的保护作用。
材料和方法
在本实验研究中，将30只成年雄性Wistar大鼠（200-250克）分为5组（n = 6）：对照组（未接受任何物质）、假手术组（仅接受纯橄榄油）、实验组1（DZN，60毫克/千克）、实验组2（维生素E，200毫克/千克）和实验组3（DZN +维生素E，相同剂量）。6周后处死所有组，使用右侧睾丸测量MDA和GSH水平。通过硫代巴比妥酸测定法测定MDA的量，使用5,5-二硫代双（2-硝基苯甲酸）（DTNB）循环法进行GSH测定。
结果
结果表明，DZN增加了MDA水平（p <0.001）并降低了GSH水平（p <0.001）。给予DZN加维生素E可降低MDA水平（p <0.001）并增加GSH水平（p = 0.001）。
结论
DZN诱导大鼠睾丸中的脂质过氧化。维生素E通过其抗氧化活性能够改善DZN的毒性作用。
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