Jagathrakshakan Sri Nisha, Sethumadhava Raghavendra Jayesh, Mehta Dhaval Tushar, Ramanathan Arvind
Department of Prosthodontia, Sree Balaji Dental College and Hospital, Bharath University, Narayanapuram, Pallikaranai, Chennai, Tamil Nadu, India.
Department of Human Genetics Laboratory, Central Research Facility, Sree Balaji Medical and Dental College and Hospital, Bharath University, Narayanapuram, Pallikaranai, Chennai, Tamil Nadu, India.
Eur J Dent. 2015 Jan-Mar;9(1):127-132. doi: 10.4103/1305-7456.149661.
To identify the prevalence of acidogenic and nonacidogenic bacteria in patients with polycaries lesions, and to ascertain caries specific bacterial prevalence in relation to noncaries controls.
Total genomic DNA extracted from saliva of three adults and four children from the same family were subjected to 16S rRNA gene sequencing analysis on a next generation sequencer, the PGS-Ion Torrent. Those bacterial genera with read counts > 1000 were considered as significant in each of the subject and used to associate the occurrence with caries.
Sequencing analysis indicated a higher prevalence of Streptococcus, Rothia, Granulicatella, Gemella, Actinomyces, Selenomonas, Haemophilus and Veillonella in the caries group relative to controls. While higher prevalence of Streptococcus, Rothia and Granulicatella were observed in all caries samples, the prevalence of others was observable in 29-57% of samples. Interestingly, Rothia and Selenomonas, which are known to occur within anaerobic environments of dentinal caries and subgingival plaque biofilms, were seen in the saliva of these caries patients. Taken together, the study has identified for the first time a unique co-prevalence pattern of bacteria in caries patients that may be explored as distinct caries specific bacterial signature to predict cariogenesis in high-risk primary and mixed dentition age groups.
确定多龋损病变患者中产酸菌和非产酸菌的患病率，并确定与无龋对照相比的龋齿特异性细菌患病率。
从同一家庭的三名成人和四名儿童的唾液中提取的总基因组DNA，在新一代测序仪PGS-Ion Torrent上进行16S rRNA基因测序分析。每个受试者中读取计数>1000的细菌属被认为具有显著性，并用于将其出现情况与龋齿相关联。
测序分析表明，与对照组相比，龋齿组中链球菌、罗氏菌、颗粒链菌、孪生球菌、放线菌、月形单胞菌、嗜血杆菌和韦荣球菌的患病率更高。虽然在所有龋齿样本中均观察到链球菌、罗氏菌和颗粒链菌的患病率较高，但其他菌的患病率在29%-57%的样本中可见。有趣的是，已知在牙本质龋和龈下菌斑生物膜的厌氧环境中出现的罗氏菌和月形单胞菌，在这些龋齿患者的唾液中也被发现。综上所述，该研究首次确定了龋齿患者中独特的细菌共患病率模式，这可能被探索为一种独特的龋齿特异性细菌特征，以预测高危乳牙列和混合牙列年龄组的龋病发生。
