Carda-Diéguez Miguel, Bravo-González Luis Alberto, Morata Isabel María, Vicente Ascensión, Mira Alex
Genomics & Health Department, FISABIO Institute, Valencia, Spain.
Department of Orthodontics, Faculty of Medicine, University of Murcia, Murcia, Spain.
J Oral Microbiol. 2019 Nov 11;12(1):1687397. doi: 10.1080/20002297.2019.1687397. eCollection 2020.
The oral microbiota has been deeply studied by high-throughput sequencing techniques. However, although the interproximal regions have one of the highest caries rates in the oral cavity, information about the bacterial composition at those sites is scarce. In this study, we used 16S rRNA Illumina sequencing to describe the microbiota associated to interproximal regions at two time points. In addition, dental plaque samples at the vestibular and lingual surfaces from the same teeth were also analysed at the two time points. Interproximal-associated microbiota was found to be similar to already described bacterial communities in other mouth niches. and were the most abundant genera in this oral region. Statistical analyses showed that the microbiota from interproximal sites was more similar to that sampled from the vestibular surfaces than to the lingual surfaces. Interestingly, many potentially cariogenic bacteria such as , or were over-represented in the interproximal regions in comparison with vestibular and lingual sites. The microbiota at interproximal regions appears to be specific and stable through time. Potentially pathogenic bacteria may increase caries development risk and gingival inflammation at those sites.
高通量测序技术已对口腔微生物群进行了深入研究。然而，尽管邻面区域是口腔中龋齿发生率最高的部位之一，但关于这些部位细菌组成的信息却很少。在本研究中，我们使用16S rRNA Illumina测序技术在两个时间点描述与邻面区域相关的微生物群。此外，还在这两个时间点对同一颗牙齿的前庭和舌面的牙菌斑样本进行了分析。发现与邻面相关的微生物群与其他口腔生态位中已描述的细菌群落相似。 和 是该口腔区域中最丰富的属。统计分析表明，来自邻面部位的微生物群与从前庭表面采集的微生物群比与舌面的更相似。有趣的是，与前庭和舌面部位相比，许多潜在致龋菌，如 、 或 在邻面区域中占比过高。邻面区域的微生物群似乎具有特异性且随时间稳定。潜在病原菌可能会增加这些部位龋齿发展风险和牙龈炎症。
