Volgenant Catherine M C, Zaura Egija, Brandt Bernd W, Buijs Mark J, Tellez Marisol, Malik Gayatri, Ismail Amid I, Ten Cate Jacob M, van der Veen Monique H
Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit, Amsterdam, the Netherlands.
Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit, Amsterdam, the Netherlands.
J Dent. 2017 Mar;58:40-47. doi: 10.1016/j.jdent.2017.01.007. Epub 2017 Jan 20.
The relation between the presence of red fluorescent plaque and the caries status in children was studied. In addition, the microbial composition of dental plaque from sites with red fluorescent plaque (RFP) and from sites with no red fluorescent plaque (NFP) was assessed.
Fluorescence photographs were taken from fifty children (6-14 years old) with overnight plaque. Full-mouth caries scores (ICDAS II) were obtained. The composition of a saliva sample and two plaque samples (RFP and NFP) was assessed using 16S rDNA sequencing.
At the site level, no clinically relevant correlations were found between the presence of RFP and the caries status. At the subject level, a weak correlation was found between RFP and the caries status when non-cavitated lesions were included (r=0.37, p=0.007). The microbial composition of RFP differed significantly from NFP. RFP had more anaerobes and more Gram-negative bacterial taxa. The most discriminative operational taxonomic units (OTUs) for RFP were Corynebacterium, Leptotrichia, Porphyromonas and Selenomonas, while the most discriminative OTUs for NFP were Neisseria, Actinomyces, Streptococcus and Rothia.
There were no clinical relevant correlations in this cross-sectional study between the presence of RFP and (early) caries lesions. There were differences in the composition of these phenotypically different plaque samples: RFP contained more Gram-negative, anaerobic taxa and was more diverse than NFP.
The study outcomes provide more insight in the possibilities to use plaque fluorescence in oral health risk assessments.
研究儿童红色荧光斑块的存在与龋齿状况之间的关系。此外，还评估了有红色荧光斑块（RFP）部位和无红色荧光斑块（NFP）部位牙菌斑的微生物组成。
对50名（6 - 14岁）有过夜牙菌斑的儿童拍摄荧光照片。获得全口龋齿评分（ICDAS II）。使用16S rDNA测序评估一份唾液样本和两份牙菌斑样本（RFP和NFP）的组成。
在部位水平上，未发现RFP的存在与龋齿状况之间存在临床相关的相关性。在个体水平上，当纳入非龋损病变时，发现RFP与龋齿状况之间存在弱相关性（r = 0.37，p = 0.007）。RFP的微生物组成与NFP有显著差异。RFP有更多的厌氧菌和更多的革兰氏阴性菌分类群。RFP最具鉴别性的可操作分类单元（OTU）是棒状杆菌属、纤毛菌属、卟啉单胞菌属和月形单胞菌属，而NFP最具鉴别性的OTU是奈瑟菌属、放线菌属、链球菌属和罗氏菌属。
在这项横断面研究中，RFP的存在与（早期）龋损病变之间不存在临床相关的相关性。这些表型不同的牙菌斑样本在组成上存在差异：RFP含有更多的革兰氏阴性厌氧菌分类群，且比NFP更多样化。
该研究结果为在口腔健康风险评估中使用牙菌斑荧光的可能性提供了更多见解。
