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[决定紫花苜蓿(Medicago sativa L.)耐盐胁迫能力的Srlk基因的天然核苷酸多态性]

[Natural nucleotide polymorphism of the Srlk gene that determines salt stress tolerance in alfalfa (Medicago sativa L)].

作者信息

Vishnevskaia M S, Pavlov A V, Dziubenko E A, Dziubenko N I, Potokina E K

出版信息

Genetika. 2014 Apr;50(4):433-42.

Abstract

Based on legume genome syntheny, the nucleotide sequence of Srlk gene, key role of which in response to salt stress was demonstrated for the model species Medicago truncatula, was identified in the major forage and siderate crop alfalfa (Medicago sativa). In twelve alfalfa samples originating from regions with contrasting growing conditions, 19 SNPs were revealed in the Srlk gene. For two nonsynonymous SNPs, molecular markers were designed that could be further used to analyze the association between Srlk gene nucleotide polymorphism and the variability in salt stress tolerance among alfalfa cultivars.

摘要

基于豆科植物基因组的共线性,在主要的饲料和绿肥作物苜蓿(紫花苜蓿)中鉴定出了Srlk基因的核苷酸序列。在模式植物蒺藜苜蓿中,该基因在响应盐胁迫方面的关键作用已得到证实。在来自生长条件差异较大地区的12个苜蓿样本中,Srlk基因中发现了19个单核苷酸多态性(SNP)。针对两个非同义SNP设计了分子标记,可进一步用于分析Srlk基因核苷酸多态性与苜蓿品种间耐盐胁迫变异性之间的关联。

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