First Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary.
First Department of Pediatrics, Semmelweis University, Budapest, Hungary.
Hypertens Res. 2015 Jun;38(6):413-8. doi: 10.1038/hr.2015.20. Epub 2015 Feb 26.
The endocannabinoid system has a key role in female reproduction, including implantation, decidualization and placentation. A growing number of studies indicate that placental and peripheral blood anandamide levels correlate closely with both spontaneous miscarriage and ectopic pregnancy. Anandamide has also been implicated in blood pressure regulation. In this study, we aimed to determine circulating anandamide levels in preeclampsia for the first time in the literature. Forty-three preeclamptic patients and 71 healthy pregnant women were involved in this case-control study. Serum anandamide concentrations were determined by high-performance liquid chromatography-mass spectrometry technique. Serum total soluble fms-like tyrosine kinase-1 (sFlt-1) and biologically active placental growth factor (PlGF) levels were measured by electrochemiluminescence immunoassay. For statistical analyses, nonparametric methods were applied. Serum levels of anandamide were significantly lower in preeclamptic patients than in healthy pregnant women (0.75 (0.44-1.03) ng ml(-1) vs. 1.30 (0.76-2.0) ng ml(-1), P<0.001). Preeclamptic patients had significantly higher sFlt-1 levels (12,121 (7963-18,316) pg ml(-1) vs. 2299 (1393-3179) pg ml(-1), P<0.001) and significantly lower PlGF concentrations (71.2 (39.2-86.4) pg ml(-1) vs. 256.8 (181.1-421.0) pg ml(-1), P<0.001) as compared with healthy pregnant women. Serum anandamide concentrations did not correlate with serum levels of sFlt-1 and PlGF in our healthy pregnant and preeclamptic groups. In conclusion, we demonstrated for the first time in the literature that serum anandamide concentrations are decreased in women with preeclampsia. However, the cause and consequence of this observation remain to be determined.
内源性大麻素系统在女性生殖中起着关键作用，包括着床、蜕膜化和胎盘形成。越来越多的研究表明，胎盘和外周血中的花生四烯酸酰胺水平与自然流产和异位妊娠密切相关。花生四烯酸酰胺也与血压调节有关。在这项研究中，我们首次旨在确定文献中子痫前期患者的循环花生四烯酸酰胺水平。本病例对照研究纳入了 43 例子痫前期患者和 71 例健康孕妇。采用高效液相色谱-质谱技术测定血清花生四烯酸酰胺浓度。采用电化学发光免疫分析法测定血清总可溶性 fms 样酪氨酸激酶-1（sFlt-1）和生物活性胎盘生长因子（PlGF）水平。统计分析采用非参数方法。与健康孕妇相比，子痫前期患者血清花生四烯酸酰胺水平显著降低（0.75（0.44-1.03）ng/ml 与 1.30（0.76-2.0）ng/ml，P<0.001）。子痫前期患者的 sFlt-1 水平明显升高（12121（7963-18316）pg/ml 与 2299（1393-3179）pg/ml，P<0.001），PlGF 浓度明显降低（71.2（39.2-86.4）pg/ml 与 256.8（181.1-421.0）pg/ml，P<0.001）。与健康孕妇相比，我们健康孕妇和子痫前期患者组血清花生四烯酸酰胺浓度与 sFlt-1 和 PlGF 血清水平无相关性。总之，我们首次在文献中证明，子痫前期妇女血清花生四烯酸酰胺浓度降低。然而，这种观察的原因和后果仍有待确定。
