Le Vu Phuong, Takatori Ryo, Iwamoto Taku, Akagi Yutaka, Satsu Hideo, Totsuka Mamoru, Chida Kazuhiro, Sato Kenji, Shimizu Makoto
Department of Applied Biological Chemistry, The University of Tokyo, Tokyo, Japan.
Skin Pharmacol Physiol. 2015;28(5):227-35. doi: 10.1159/000369830. Epub 2015 Feb 14.
Oral ingestion of collagen peptides (CP) has long been suggested to exert beneficial effects on the skin, but the molecular events induced by CP on the skin remain unclear. Here, we investigated the effects of oral CP administration on gene expression in hairless mouse skin and of prolyl-hydroxyproline (Pro-Hyp), a collagen-derived dipeptide, on gene expression in a coculture of mouse skin keratinocytes and fibroblasts. Using microarray analysis, we found that oral administration of CP to hairless mice for 6 weeks induced increased expression of Krtap and Krt genes in the skin. Annotation analysis using DAVID revealed that a group of the up-regulated genes, Gprc5d, Sprr2a1, Krt27 and Krtap16-7, is associated with the development of the epidermis and the hair cycle. In addition, the presence of Pro-Hyp (200 μM) induced an increase in the expression of Krtap16-7, Krtap15, Krtap14 and Krtap8-2 in keratinocytes in coculture, partially resembling the in vivo result. The Pro-Hyp-induced up-regulation of these genes was not observed when keratinocytes were cultured without fibroblasts, suggesting that the presence of fibroblasts is essential for the effects of Pro-Hyp. Our study presents new insights into the effects of CP on the skin, which might link to the hair cycle.
长期以来，人们一直认为口服胶原蛋白肽（CP）对皮肤有有益作用，但CP在皮肤上诱导的分子事件仍不清楚。在这里，我们研究了口服CP对无毛小鼠皮肤基因表达的影响，以及胶原蛋白衍生的二肽脯氨酰羟脯氨酸（Pro-Hyp）对小鼠皮肤角质形成细胞和成纤维细胞共培养物中基因表达的影响。使用微阵列分析，我们发现给无毛小鼠口服CP 6周可诱导皮肤中Krtap和Krt基因表达增加。使用DAVID进行的注释分析表明，一组上调基因Gprc5d、Sprr2a1、Krt27和Krtap16-7与表皮发育和毛发周期有关。此外，Pro-Hyp（200 μM）的存在诱导共培养的角质形成细胞中Krtap16-7、Krtap15、Krtap14和Krtap8-2的表达增加，部分类似于体内结果。当角质形成细胞在没有成纤维细胞的情况下培养时，未观察到Pro-Hyp诱导的这些基因上调，这表明成纤维细胞的存在对Pro-Hyp的作用至关重要。我们的研究为CP对皮肤的影响提供了新的见解，这可能与毛发周期有关。
