Haratake Akinori, Watase Daisuke, Setoguchi Shuichi, Nagata-Akaho Nami, Matsunaga Kazuhisa, Takata Jiro
Laboratory of Drug Design and Drug Delivery, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
Laboratory of Drug Design and Drug Delivery, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
Life Sci. 2017 Apr 1;174:77-82. doi: 10.1016/j.lfs.2017.02.013. Epub 2017 Mar 1.
Influence on collagen content with oral ingestion of diosgenin (Dios) was investigated in established low collagen skin mouse model. And its mechanism of action was investigated using primary cultured fibroblasts.
Hairless mice were fed a low protein diet with Dios for 8weeks and the contents of collagen in skin were determined by measuring the content of hydroxyproline (Hyp). In primary cultured fibroblasts, the numbers of fibroblast were determined by incubating with Dios for 120h; the contents of Hyp were determined by incubating with Dios for 24 or 72h using fibroblasts of confluent state; the expressions of messenger ribonucleic acid (mRNA) were determined by incubating with Dios for 24h.
Oral ingestion of Dios in the diet for 8weeks led to a dose-dependent increase in the Hyp content as collagen content of skin. In proliferating of primary cultured fibroblasts, Dios treatment led to a decrease of adenosine 5'-triphosphate content indicating decrease of the cell number. In the cells reached to confluent, although increase of Hyp content in the control indicating progress of fibroblasts differentiation were observed, the content of Hyp remained unchanged with Dios treatment. Finally, addition of Dios led to a decrease the α-tubulin and c-fos mRNA expressions relating to the cell cycle.
It is concluded that Dios can improve skin collagen content by shifting the dynamics of the fibroblasts from proliferation to differentiation via cell cycle arrest.
在已建立的低胶原蛋白皮肤小鼠模型中研究口服薯蓣皂苷元（Dios）对胶原蛋白含量的影响。并使用原代培养的成纤维细胞研究其作用机制。
无毛小鼠用含Dios的低蛋白饮食喂养8周，通过测量羟脯氨酸（Hyp）含量来测定皮肤中胶原蛋白的含量。在原代培养的成纤维细胞中，用Dios孵育120小时后测定成纤维细胞数量；使用汇合状态的成纤维细胞，用Dios孵育24或72小时后测定Hyp含量；用Dios孵育24小时后测定信使核糖核酸（mRNA）的表达。
饮食中口服Dios 8周导致皮肤胶原蛋白含量中Hyp含量呈剂量依赖性增加。在原代培养的成纤维细胞增殖过程中，Dios处理导致三磷酸腺苷含量降低，表明细胞数量减少。在细胞达到汇合状态时，尽管观察到对照组中Hyp含量增加表明成纤维细胞分化进展，但Dios处理后Hyp含量保持不变。最后，添加Dios导致与细胞周期相关的α-微管蛋白和c-fos mRNA表达降低。
得出结论，Dios可通过使成纤维细胞动力学从增殖转变为分化，通过细胞周期停滞来改善皮肤胶原蛋白含量。
