Galli Lorenzo, Son Sang Kil, White Thomas A, Santra Robin, Chapman Henry N, Nanao Max H
Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
EMBL, Grenoble Outstation, Rue Jules Horowitz 6, 38042 Grenoble, France.
J Synchrotron Radiat. 2015 Mar;22(2):249-55. doi: 10.1107/S1600577514027854. Epub 2015 Feb 5.
Here, it is shown that simulated native serial femtosecond crystallography (SFX) cathepsin B data can be phased by rapid ionization of sulfur atoms. Utilizing standard software adopted for radiation-damage-induced phasing (RIP), the effects on both substructure determination and phasing of the number of collected patterns and fluences are explored for experimental conditions already available at current free-electron laser facilities.
在此，研究表明，通过硫原子的快速电离可以对模拟的天然序列飞秒晶体学（SFX）组织蛋白酶B数据进行相位分析。利用用于辐射损伤诱导相位分析（RIP）的标准软件，针对当前自由电子激光设施已具备的实验条件，探讨了收集的图案数量和注量对亚结构确定和相位分析的影响。
