Structural Biology Group, European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38000 Genoble, France.
Center for Free-Electron Laser Science, Deutsches Elektronensynchrotron, Notkestrasse 85, 22607 Hamburg, Germany.
Acta Crystallogr D Struct Biol. 2018 Apr 1;74(Pt 4):366-378. doi: 10.1107/S2059798318001535. Epub 2018 Apr 6.
Specific radiation damage can be used to determine phases de novo from macromolecular crystals. This method is known as radiation-damage-induced phasing (RIP). One limitation of the method is that the dose of individual data sets must be minimized, which in turn leads to data sets with low multiplicity. A solution to this problem is to use data from multiple crystals. However, the resulting signal can be degraded by a lack of isomorphism between crystals. Here, it is shown that serial synchrotron crystallography in combination with selective merging of data sets can be used to determine high-quality phases for insulin and thaumatin, and that the increased multiplicity can greatly enhance the success rate of the experiment.
特定的辐射损伤可用于从头确定大分子晶体的相。这种方法被称为辐射损伤诱导相（RIP）。该方法的一个限制是必须最小化各个数据集的剂量，这反过来又导致多重性低的数据集。解决此问题的一种方法是使用多个晶体的数据。但是，晶体之间缺乏同构性会降低信号质量。本文表明，结合同步加速器晶体学和数据集的选择性合并，可以用于确定胰岛素和天花粉蛋白的高质量相，并且增加的多重性可以大大提高实验的成功率。
