Department of Plastic and Cosmetic Surgery, the Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang Province, China.
Adv Skin Wound Care. 2010 Jan;23(1):29-33. doi: 10.1097/01.ASW.0000363486.94352.41.
Clinical observations indicate that the irradiation of pulsed-dye laser (PDL) can inhibit keloid growth; however, the mechanism of this process is unknown. The aim of this study was to explore the molecular mechanism of the action of PDL on keloid fibroblasts.
Twenty patients with keloids were selected for this study. Fibroblasts were harvested from keloid tissue. Cell cycle distribution and apoptosis induction were analyzed by flow cytometry and with an antibody to Fas. The expression of apoptosis-related proteins (Fas, BCL-2, and p53) was measured by flow cytometry.
Fibroblasts with laser irradiation and control fibroblasts displayed significant resistance to Fas-mediated apoptosis. Analysis of cell cycle distribution indicated that approximately 64% of control fibroblasts were in the proliferative periods of the cell cycle (G2 to M and S phases). However, most fibroblasts with laser irradiation were in the G0 to G1 phase. Fas and BCL-2 expression did not differ significantly between the 2 groups, but p53 expression was much higher in fibroblasts with laser irradiation than in control fibroblasts.
It is suggested that differences in cell cycle distribution and p53 protein expression may partly account for the biologic mechanism of 595-nm PDL in treating keloid disease.
临床观察表明，脉冲染料激光（PDL）的辐照可以抑制瘢痕疙瘩的生长；然而，其作用机制尚不清楚。本研究旨在探讨 PDL 作用于瘢痕疙瘩成纤维细胞的分子机制。
本研究选取 20 例瘢痕疙瘩患者，从瘢痕疙瘩组织中分离出成纤维细胞。通过流式细胞术和 Fas 抗体分析细胞周期分布和诱导细胞凋亡，通过流式细胞术检测凋亡相关蛋白（Fas、BCL-2 和 p53）的表达。
激光照射组和成纤维细胞对照组的成纤维细胞对 Fas 介导的凋亡具有显著的抗性。细胞周期分布分析表明，对照组约有 64%的成纤维细胞处于细胞周期的增殖期（G2 期至 M 期和 S 期）。然而，大多数激光照射组的成纤维细胞处于 G0 期至 G1 期。两组 Fas 和 BCL-2 的表达无明显差异，但激光照射组的 p53 表达明显高于对照组。
提示细胞周期分布和 p53 蛋白表达的差异可能部分解释了 595nm PDL 治疗瘢痕疙瘩疾病的生物学机制。
