Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB Oxford, UK.
The Genome Analysis Centre, Norwich Research Park, NR4 7UH Norwich, UK.
J Mol Biol. 2015 Jul 31;427(15):2414-2417. doi: 10.1016/j.jmb.2015.02.018. Epub 2015 Feb 26.
Circular RNAs are found in a wide range of organisms and it has been proposed that they perform disparate functions. However, how RNA circularization is connected to alternative splicing remains largely unexplored. Here, we stimulated primary human endothelial cells with tumor necrosis factor α or tumor growth factor β, purified RNA, generated >2.4 billion RNA-seq reads, and used a custom pipeline to characterize circular RNAs derived from coding exons. We find that circularization of exons is widespread and correlates with exon skipping, a feature that adds considerably to the regulatory complexity of the human transcriptome.
环状 RNA 存在于广泛的生物中，有人提出它们具有不同的功能。然而，RNA 环化与选择性剪接的关系在很大程度上仍未得到探索。在这里，我们用肿瘤坏死因子 α或肿瘤生长因子 β刺激原代人内皮细胞，纯化 RNA，生成超过 24 亿个 RNA-seq 读段，并使用定制管道来描述来源于编码外显子的环状 RNA。我们发现外显子的环化很普遍，并且与外显子跳跃相关，这一特征极大地增加了人类转录组的调控复杂性。
