用于多光子显微镜的基于飞秒二极管的时间透镜激光器。
Femtosecond diode-based time lens laser for multiphoton microscopy.
作者信息
Lange Simmons Y, Underwood Kenneth J, Supekar Omkar D, Heffernan Brendan M, Welton Tarah A, Gibson Emily A, Gopinath Juliet T
机构信息
Department of Physics, University of Colorado, Boulder, CO 80309, USA.
Department of Mechanical Engineering, University of Colorado, Boulder, CO 80309, USA.
出版信息
Biomed Opt Express. 2021 Sep 14;12(10):6269-6276. doi: 10.1364/BOE.430549. eCollection 2021 Oct 1.
We demonstrate a near-infrared, femtosecond, diode laser-based source with kW peak power for two-photon microscopy. At a wavelength of 976 nm, the system produces sub-ps pulses operating at a repetition rate of 10 MHz with kilowatt class peak powers suitable for deep tissue two-photon microscopy. The system, integrated with a laser-scanning microscope, images to a depth of 900 µm in a fixed sample of PLP-eGFP labeled mouse brain tissue. This represents a significant development that will lead to more efficient, compact, and accessible laser sources for biomedical imaging.
我们展示了一种基于近红外飞秒二极管激光器的光源，其峰值功率可达千瓦级，用于双光子显微镜。在976纳米波长下，该系统产生亚皮秒脉冲，重复频率为10兆赫兹，峰值功率为千瓦级，适用于深部组织双光子显微镜。该系统与激光扫描显微镜集成，在PLP-eGFP标记的小鼠脑组织固定样本中成像深度可达900微米。这是一项重大进展，将为生物医学成像带来更高效、紧凑且易于使用的激光源。
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