一种新建立的用于检测蓖麻硬蜱中宫本疏螺旋体的实时聚合酶链反应。
A newly established real-time PCR for detection of Borrelia miyamotoi in Ixodes ricinus ticks.
作者信息
Reiter Michael, Schötta Anna-Margarita, Müller Andreas, Stockinger Hannes, Stanek Gerold
机构信息
Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090 Vienna, Austria.
Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Kinderspitalgasse 15, 1090 Vienna, Austria.
出版信息
Ticks Tick Borne Dis. 2015 Apr;6(3):303-8. doi: 10.1016/j.ttbdis.2015.02.002. Epub 2015 Feb 23.
A total of 350 ticks collected in Austria were analyzed for the presence of DNA sequences of B. miyamotoi. Three ticks gave positive results in a B. miyamotoi-specific nested PCR. Results were confirmed by sequencing the amplified glpQ gene from the positive samples. Moreover we developed a real-time PCR which unambiguously detected B. miyamotoi in all positive samples. Further genotyping of the samples found 100% identity of the 16S-23S intergenic spacer region with Swedish B. miyamotoi sequences. This is the first detection of the relapsing fever spirochete Borrelia miyamotoi in hard ticks in Austria. The results consolidate the picture of a European-wide distribution of B. miyamotoi and again underscore the need for clinical awareness to clarify possible involvement of this species in human disease.
对在奥地利采集的350只蜱虫进行了分析，以检测是否存在宫本疏螺旋体的DNA序列。在宫本疏螺旋体特异性巢式PCR中，有3只蜱虫检测结果呈阳性。通过对阳性样本中扩增出的glpQ基因进行测序，证实了结果。此外，我们开发了一种实时PCR方法，可在所有阳性样本中明确检测出宫本疏螺旋体。对样本进一步进行基因分型发现，16S - 23S基因间隔区与瑞典宫本疏螺旋体序列的一致性为100%。这是在奥地利硬蜱中首次检测到复发性发热螺旋体——宫本疏螺旋体。这些结果巩固了宫本疏螺旋体在欧洲广泛分布的情况，并再次强调了临床意识的必要性，以明确该物种可能与人类疾病的关联。
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