Ren Hailin, Li Xiaona, Cheng Guojun, Li Ning, Hou Zhi, Suo Jiming, Wang Jian, Za Xi
Department of Urology, Affiliated Hospital of Qinghai University , Xining , PR China and.
Aging Male. 2015 Jun;18(2):84-8. doi: 10.3109/13685538.2015.1018159. Epub 2015 Mar 9.
The objective of this study is to explore the effects of reactive oxygen species (ROS) under hypoxic environment in prostatic stromal cells (PSC).
To detect the expression of ROS in PSC and the tissues of benign prostatic hyperplasia (BPH) by flow cytometry; under hypoxic conditions, to observe the changes of cells growth and ROS in PSC; quantitative PCR was used to detect hypoxia inducible factor-1α (HIF-1α), androgen receptors (AR), vascular endothelial growth factor (VEGF), and interleukin-8 (IL-8) in PSC; After edaravone intervening, to examine the changes of cells growth, ROS, HIF-1α, AR, VEGF, and IL-8 under hypoxic conditions.
The expression of ROS in tissues and cells which under hypoxic condition was significantly increased. 3% O2 promoted the proliferation. The HIF-1α, AR, VEGF, and IL-8 were upregulated under 3% O2. After edaravone intervening, ROS significantly decreased, HIF-1α and VEGF were downregulated, and cell proliferation declined.
Hypoxia stimulates the generation of ROS, and the ROS may play a key role in BPH.
本研究旨在探讨低氧环境下活性氧（ROS）在前列腺基质细胞（PSC）中的作用。
采用流式细胞术检测PSC及良性前列腺增生（BPH）组织中ROS的表达；在低氧条件下，观察PSC细胞生长及ROS的变化；运用定量PCR检测PSC中缺氧诱导因子-1α（HIF-1α）、雄激素受体（AR）、血管内皮生长因子（VEGF）和白细胞介素-8（IL-8）；依达拉奉干预后，检测低氧条件下细胞生长、ROS、HIF-1α、AR、VEGF和IL-8的变化。
低氧条件下组织和细胞中ROS表达显著增加。3%氧气促进细胞增殖。在3%氧气条件下，HIF-1α、AR、VEGF和IL-8上调。依达拉奉干预后，ROS显著降低，HIF-1α和VEGF下调，细胞增殖下降。
低氧刺激ROS生成，ROS可能在BPH中起关键作用。
