脂质相关抗氧化剂：初治良性前列腺增生患者中的芳基酯酶和对氧磷酶-1
Lipid associated antioxidants: arylesterase and paraoxonase-1 in benign prostatic hyperplasia treatment-naïve patients.
作者信息
Asare George Awuku, Andam Sabina Ekua, Asare-Anane Henry, Ammanquah Seth, Anang-Quartey Yvonne, Afriyie Daniel K, Musah Iddis
机构信息
Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Korle Bu Campus, Ghana.
Department of Chemical Pathology, School of Biomedical and Allied Health Sciences, University of Ghana, Korle Bu Campus, Ghana.
出版信息
Prostate Int. 2018 Mar;6(1):36-40. doi: 10.1016/j.prnil.2017.04.002. Epub 2017 Apr 20.
BACKGROUND
Oxidative stress and antioxidants have been implicated in many diseases including prostate cancer and benign prostatic hyperplasia (BPH). Lipid peroxidation contributes to oxidative stress. However, new and emerging antioxidants such as paraoxonase 1 (PON1) and arylesterase (ARE) associated with lipoprotein peroxidation have not been examined in BPH patients. PON1 and ARE, a high-density lipoprotein (HDL) cholesterol-bound enzyme system of antioxidants, protect low-density lipoprotein (LDL) cholesterol and HDL from oxidation by hydrolysis. The study primarily determined paraoxonase (PON1) and ARE activities in BPH treatment-naïve patients.
MATERIALS AND METHODS
Sixty newly diagnosed patients (treatment-naïve) alongside 30 apparently healthy controls were recruited. Blood examinations included lipid profile (total cholesterol, triglycerides, LDL, HDL), glutathione peroxidase, PON1, ARE, and prostate specific antigen (PSA).Prostate volume and International Prostate Symptoms Score (IPSS) were determined.
RESULTS
PSA was significantly different between patient and control groups ( < 0.0001). Total cholesterol, triglycerides, and LDL were significantly higher in the patient group ( = 0.002, < 0.001, = 0.003, respectively). Glutathione peroxidase was very low in the patient group compared to the control group (5.65 ± 2.30 ng/mL and 17.43 ± 10.98 ng/mL, respectively). Although PON1 was higher in the patient group (50.22 ± 19.68/61.30 ± 29.55 ng/mL; > 0.05), ARE was significantly lower in the patient group (61.31 ± 21.76/49.30 ± 19.82 ng/mL; = 0.0098). No correlation was established between antioxidants and the lipid profile except for the LDL and PON1 patient group (r = 0.1486, = 0.0374). Similarly, a weak correlation was also established between PSA and LDL in the patient group (r = -0.275, = 0.033). PON1/HDL ratio was not significantly different. However, the ARE/HDL ratio was significantly lower in the patient group ( < 0.0001).
CONCLUSION
These results signify the presence of a higher lipoprotein peroxidation activity and lower lipid-associated antioxidant activity in the patient group. The ARE/HDL ratio is a better indicator of the HDL associated antioxidant than the PON1/HDL ratio or the individual antioxidants (PON1 and ARE) as reported by others.
背景
氧化应激和抗氧化剂与包括前列腺癌和良性前列腺增生（BPH）在内的多种疾病有关。脂质过氧化会导致氧化应激。然而，新出现的抗氧化剂如对氧磷酶1（PON1）和芳基酯酶（ARE）与脂蛋白过氧化有关，尚未在BPH患者中进行研究。PON1和ARE是一种与高密度脂蛋白（HDL）胆固醇结合的抗氧化酶系统，通过水解作用保护低密度脂蛋白（LDL）胆固醇和HDL不被氧化。该研究主要测定了未经治疗的BPH患者的对氧磷酶（PON1）和ARE活性。
材料与方法
招募了60名新诊断的（未经治疗的）患者以及30名明显健康的对照者。血液检查包括血脂谱（总胆固醇、甘油三酯、LDL、HDL）、谷胱甘肽过氧化物酶、PON1、ARE和前列腺特异性抗原（PSA）。测定了前列腺体积和国际前列腺症状评分（IPSS）。
结果
患者组和对照组之间的PSA有显著差异（<0.0001）。患者组的总胆固醇、甘油三酯和LDL显著更高（分别为=0.002，<0.001，=0.003）。与对照组相比，患者组的谷胱甘肽过氧化物酶非常低（分别为5.65±2.30 ng/mL和17.43±10.98 ng/mL）。虽然患者组的PON1较高（50.22±19.68/61.30±29.55 ng/mL；>0.05），但患者组的ARE显著更低（61.31±21.76/49.30±19.82 ng/mL；=0.0098）。除了患者组的LDL和PON1之间（r=0.1486，=0.0374），抗氧化剂与血脂谱之间未建立相关性。同样，患者组中PSA和LDL之间也建立了弱相关性（r=-0.275，=0.033）。PON1/HDL比值无显著差异。然而，患者组的ARE/HDL比值显著更低（<0.0001）。
结论
这些结果表明患者组存在较高的脂蛋白过氧化活性和较低的脂质相关抗氧化活性。如其他人所报道，ARE/HDL比值比PON1/HDL比值或单个抗氧化剂（PON1和ARE）更能作为HDL相关抗氧化剂的指标。
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