中国中部地区宠物狗弓形虫的流行情况及基因特征
Prevalence and genetic characterization of Toxoplasma gondii in pet dogs in Central China.
作者信息
Qian Wei-Feng, Yan Wen-Chao, Wang Tian-Qi, Zhai Kai, Han Li-Fang, Lv Chao-Chao
机构信息
College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.
PuLike Biological Engineering Co., Ltd, Luoyang 471000, China.
出版信息
Korean J Parasitol. 2015 Feb;53(1):125-8. doi: 10.3347/kjp.2015.53.1.125. Epub 2015 Feb 27.
The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10.4% (13/125) in PCR, respectively. No statistical associations were found between animal gender and age and the prevalence of T. gondii infection. Thirteen positive DNA samples were genotyped using 11 PCR-RFLP markers, including SAG1, (3'+5') SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. Of these, only 2 samples were genotyped with complete data for all loci, and a novel genotype (type III at SAG3 and GRA6 loci, and type I at other loci) was identified. This is the first report of genetic characterization of T. gondii infection in dogs in China.
对中国中部河南省犬类弓形虫感染的患病率和基因型进行了调查。2013年4月至6月期间，共采集了125份宠物犬血样，所有样本均通过间接血凝抗体试验（IHA）和巢式PCR进行检测。宠物犬中弓形虫的总体患病率为24.0%（30/125），IHA检测患病率为20.8%（26/125），PCR检测患病率为10.4%（13/125）。未发现动物性别和年龄与弓形虫感染患病率之间存在统计学关联。使用包括SAG1、(3'+5') SAG2、alt.SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和Apico在内的11个PCR-RFLP标记对13个阳性DNA样本进行基因分型。其中，只有2个样本获得了所有位点的完整基因分型数据，并鉴定出一种新的基因型（SAG3和GRA6位点为III型，其他位点为I型）。这是中国犬类弓形虫感染基因特征的首次报道。
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