Jin Jinpu, He Kun, Tang Xing, Li Zhe, Lv Le, Zhao Yi, Luo Jingchu, Gao Ge
State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Center for Bioinformatics, Peking University, Beijing, P.R. China.
Monsanto Biotechnology R&D Center, Beijing, P.R. China.
Mol Biol Evol. 2015 Jul;32(7):1767-73. doi: 10.1093/molbev/msv058. Epub 2015 Mar 6.
Transcription factors (TFs) play key roles in both development and stress responses. By integrating into and rewiring original systems, novel TFs contribute significantly to the evolution of transcriptional regulatory networks. Here, we report a high-confidence transcriptional regulatory map covering 388 TFs from 47 families in Arabidopsis. Systematic analysis of this map revealed the architectural heterogeneity of developmental and stress response subnetworks and identified three types of novel network motifs that are absent from unicellular organisms and essential for multicellular development. Moreover, TFs of novel families that emerged during plant landing present higher binding specificities and are preferentially wired into developmental processes and these novel network motifs. Further unveiled connection between the binding specificity and wiring preference of TFs explains the wiring preferences of novel-family TFs. These results reveal distinct functional and evolutionary features of novel TFs, suggesting a plausible mechanism for their contribution to the evolution of multicellular organisms.
转录因子（TFs）在发育和应激反应中都起着关键作用。通过整合并重塑原始系统，新型转录因子对转录调控网络的进化有显著贡献。在此，我们报告了一张高可信度的转录调控图谱，涵盖拟南芥中47个家族的388个转录因子。对该图谱的系统分析揭示了发育和应激反应子网络的结构异质性，并鉴定出三种新型网络基序，这些基序在单细胞生物中不存在，但对多细胞发育至关重要。此外，在植物登陆过程中出现的新型家族的转录因子具有更高的结合特异性，并优先连接到发育过程和这些新型网络基序中。进一步揭示的转录因子结合特异性与连接偏好之间的联系解释了新型家族转录因子的连接偏好。这些结果揭示了新型转录因子独特的功能和进化特征，为它们对多细胞生物进化的贡献提供了一种合理的机制。
