Piletska Elena V, Abd Bashar H, Krakowiak Agata S, Parmar Anitha, Pink Demi L, Wall Katie S, Wharton Luke, Moczko Ewa, Whitcombe Michael J, Karim Kal, Piletsky Sergey A
Department of Chemistry, College of Science and Engineering, University of Leicester, LE1 7RH, UK.
Analyst. 2015 May 7;140(9):3113-20. doi: 10.1039/c4an02292k. Epub 2015 Mar 9.
Curcumin is a versatile anti-inflammatory and anti-cancer agent known for its low bioavailability, which could be improved by developing materials capable of binding and releasing drug in a controlled fashion. The present study describes the preparation of magnetic nano-sized Molecularly Imprinted Polymers (nanoMIPs) for the controlled delivery of curcumin and their high throughput characterisation using microtitre plates modified with magnetic inserts. NanoMIPs were synthesised using functional monomers chosen with the aid of molecular modelling. The rate of release of curcumin from five polymers was studied under aqueous conditions and was found to correlate well with the binding energies obtained computationally. The presence of specific monomers was shown to be significant in ensuring effective binding of curcumin and to the rate of release obtained. Characterisation of the polymer particles was carried out using dynamic light scattering (DLS) technique and scanning electron microscopy (SEM) in order to establish the relationship between irradiation time and particle size. The protocols optimised during this study could be used as a blueprint for the development of nanoMIPs capable of the controlled release of potentially any compound of interest.
姜黄素是一种用途广泛的抗炎和抗癌药物，但其生物利用度较低，可通过开发能够以可控方式结合和释放药物的材料来提高。本研究描述了用于姜黄素控释的磁性纳米尺寸分子印迹聚合物（nanoMIPs）的制备及其使用磁插入物修饰的微量滴定板进行的高通量表征。nanoMIPs是借助分子建模选择功能单体合成的。研究了姜黄素在五种聚合物中的水相释放速率，发现其与通过计算获得的结合能密切相关。结果表明特定单体的存在对于确保姜黄素的有效结合及其释放速率具有重要意义。使用动态光散射（DLS）技术和扫描电子显微镜（SEM）对聚合物颗粒进行表征，以建立辐照时间与粒径之间的关系。本研究中优化的方案可作为开发能够潜在地控释任何感兴趣化合物的nanoMIPs的蓝图。
