Sáiz Jorge, Koenka Israel Joel, García-Ruiz Carmen, Müller Beat, Chwalek Thomas, Hauser Peter C
Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá, Alcalá de Henares, Madrid, Spain.
University Institute of Research in Police Sciences (IUICP), University of Alcalá, Alcalá de Henares, Madrid, Spain.
Electrophoresis. 2015 Aug;36(16):1941-4. doi: 10.1002/elps.201400589. Epub 2015 Apr 2.
A novel micro-injector for capillary electrophoresis for the handling of samples with volumes down to as little as 300 nL was designed and built in our laboratory for analyses in which the available volume is a limitation. The sample is placed into a small cavity located directly in front of the separation capillary, and the injection is then carried out automatically by controlled pressurization of the chamber with compressed air. The system also allows automated flushing of the injection chamber as well as of the capillary. In a trial with a capillary electrophoresis system with contactless conductivity detector, employing a capillary of 25 μm diameter, the results showed good stability of migration times and peak areas. To illustrate the technique, the fast separation of five inorganic cations (Na(+) , K(+) , NH4 (+) , Ca(2+) , and Mg(2+) ) was set up. This could be achieved in less than 3 min, with good limits of detection (10 μM) and linear ranges (between about 10 and 1000 μM). The system was demonstrated for the determination of the inorganic cations in porewater samples of a lake sediment core.
我们实验室设计并制造了一种用于毛细管电泳的新型微量注射器，用于处理低至300 nL体积的样品，适用于样品体积有限的分析。将样品置于直接位于分离毛细管前方的小腔中，然后通过用压缩空气对腔室进行控制加压来自动进行进样。该系统还允许对进样腔室以及毛细管进行自动冲洗。在使用具有非接触式电导检测器的毛细管电泳系统进行的试验中，采用直径为25μm的毛细管，结果表明迁移时间和峰面积具有良好的稳定性。为了说明该技术，建立了五种无机阳离子（Na(+)、K(+)、NH4(+)、Ca(2+)和Mg(2+)）的快速分离方法。这可以在不到3分钟的时间内实现，具有良好的检测限（10μM）和线性范围（约10至1000μM之间）。该系统已用于测定湖泊沉积物岩芯孔隙水样品中的无机阳离子。
