Department of Chemistry, University of Basel, Basel, Switzerland.
Electrophoresis. 2019 Feb;40(3):410-413. doi: 10.1002/elps.201800250. Epub 2018 Sep 2.
A fast automated system for rapid electrophoretic separations in short conventional capillaries employing contactless conductivity detection is presented. The instrument is based on pneumatic pressurization and does not require a conventional pump. The required pressures and flow rates for the different steps of the injection and flushing processes are produced with the help of two flow restrictors. The device is implemented on a microfluidic breadboard with dimensions of ca. 13 × 20 cm and employs miniature valves. Nine inorganic cations, namely NH K , Na , Ca , Mg , Mn , Sr , Li , and Ba , could be separated in a capillary of 10 μm inner diameter and 6 cm effective length within 25 s. Following a reduction of the effective length to 4 cm, still five inorganic cations could be separated in a time span of 12 s. The repeatability of peak areas was better than 3.1 % and limits of detection between 3.5 and 5.5 μM were achieved.
一种快速自动化系统，用于在短常规毛细管中进行快速电泳分离，采用非接触式电导检测。该仪器基于气动加压，不需要传统的泵。通过两个流量限制器的帮助，可以产生注入和冲洗过程的不同步骤所需的压力和流速。该设备基于微流控实验板实现，尺寸约为 13×20 厘米，并采用微型阀门。在 10μm 内径和 6cm 有效长度的毛细管中，可以在 25 秒内分离 9 种无机阳离子，即 NH₄、K、Na、Ca、Mg、Mn、Sr、Li 和 Ba。将有效长度减小到 4cm 后，仍可在 12s 内分离出 5 种无机阳离子。峰面积的重复性优于 3.1%，检测限在 3.5 至 5.5μM 之间。
