Balázs M, Illyés G, Vadász G
Department of Pathology, János Hospital, Budapest, Hungary.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;57(6):353-60. doi: 10.1007/BF02899101.
The changes in the number and ultrastructure of mast cells were studied in 37 colonoscopical biopsies from patients with ulcerative colitis. Changes in the active stage of the disease and during remission were compared. Cell counts were performed on semithin sections stained with Giemsa after osmium tetroxide fixation. This method overcome the uncertain staining found after formalin fixation. Accumulation of mast cells accompanied by intense degranulation was found to be significant in the active stage of the disease. Two forms of degranulation were observed: discharge of the individual granules and protrusion and detachment of the cytoplasmic processes containing granules. The latter was a sign of rapid degranulation, as described earlier in animal experiments. Mast cells were closely associated with capillary blood vessels, Schwann cells, neural fibres, myofibroblasts and collagenous fibres, and were also present between epithelial cells. It is assumed that close topographic contact may also imply a functional correlation.
对37例溃疡性结肠炎患者的结肠镜活检组织进行了肥大细胞数量及超微结构变化的研究。比较了疾病活动期和缓解期的变化。在四氧化锇固定后，对用吉姆萨染色的半薄切片进行细胞计数。该方法克服了福尔马林固定后出现的染色不确定问题。发现在疾病活动期，肥大细胞积聚并伴有强烈脱颗粒现象显著。观察到两种脱颗粒形式：单个颗粒的释放以及含颗粒的细胞质突起的突出和脱离。后者是快速脱颗粒的标志，如先前在动物实验中所描述的。肥大细胞与毛细血管、施万细胞、神经纤维、肌成纤维细胞和胶原纤维密切相关，也存在于上皮细胞之间。推测紧密的拓扑接触可能也意味着功能相关性。
