Rijzewijk J J, Van Erp P E, Bauer F W
Department of Dermatology, University Hospital, Nijmegen, The Netherlands.
Acta Derm Venereol. 1989;69(6):512-5.
The monoclonal antibody Ki67 (Ki67) binds to a nuclear antigen expressed by cycling cells of several human tissues and to the cytoplasm of the basal layer cells of squamous epithelia. We have used an immunohistochemical method to visualize the binding sites of Ki67 in normal and hyperproliferative epidermis. Cytoplasmic staining was present in the basal layer cells of normal epidermis, but was decreased in psoriatic and post-tapestripping epidermis. In sections of normal epidermis only a small minority of nuclei were positive, but sections of psoriatic epidermis and epidermis 40 and 48 h after tapestripping showed large numbers of positive nuclei in the basal and suprabasal layers. Since recent reports strongly suggest that the cell production rate is regulated by changes in the number of cycling cells, the hypothesis that Ki67 binds also in human epidermis to the nuclei of cycling cells is supported by the present findings.
单克隆抗体Ki67（Ki67）可与几种人体组织中处于细胞周期的细胞所表达的一种核抗原以及鳞状上皮基底层细胞的细胞质结合。我们采用免疫组织化学方法来观察Ki67在正常和增殖过度的表皮中的结合位点。正常表皮的基底层细胞存在细胞质染色，但在银屑病表皮和胶带剥离后的表皮中这种染色减少。在正常表皮切片中只有少数细胞核呈阳性，但银屑病表皮切片以及胶带剥离后40小时和48小时的表皮切片显示，基底层和基底上层有大量阳性细胞核。由于近期报告有力地表明细胞产生率受处于细胞周期的细胞数量变化的调节，目前的研究结果支持了Ki67在人体表皮中也与处于细胞周期的细胞的细胞核结合这一假说。
