van der Vleuten C J, Kroot E J, de Jong E M, van de Kerkhof P C
University Hospital Nijmegen, Department of Dermatology, The Netherlands.
Arch Dermatol Res. 1996 Aug;288(9):510-6. doi: 10.1007/BF02505246.
Ultraviolet B (UVB) irradiation has extensively been advocated for use in the investigation of cutaneous inflammation in vivo. Mostly doses above the threshold of skin damage have been used. Therefore it is not clear whether the changes observed are specific effects of UVB or to a certain extent represent wound healing. In this study the dose-dependent effects of UVB on normal human skin were assessed using histology and immunohistochemistry. The dose of 1 MED was chosen as a dose unducing tissue changes with adequate morphology: no toxicity but evident immunohistochemical changes. The sequential effects of this 1 MED of UVB were studied for up to 14 days after irradiation, using immunohistochemistry with a panel of monoclonal antibodies. Substantial effects were observed, mainly on proliferation and differentiation; the markers for inflammation did not reveal major changes. This model might be a promising approach to evaluate the effect of drugs on epidermal proliferation and differentiation in vivo.
紫外线B（UVB）照射已被广泛提倡用于体内皮肤炎症的研究。大多使用的剂量高于皮肤损伤阈值。因此，尚不清楚观察到的变化是UVB的特定效应还是在一定程度上代表伤口愈合。在本研究中，使用组织学和免疫组织化学评估UVB对正常人类皮肤的剂量依赖性效应。选择1个最小红斑量（MED）作为诱导具有适当形态学的组织变化的剂量：无毒性但有明显的免疫组织化学变化。使用一组单克隆抗体进行免疫组织化学，研究照射后长达14天的这1个MED的UVB的连续效应。观察到了显著效应，主要是对增殖和分化的影响；炎症标志物未显示出主要变化。该模型可能是一种有前景的方法，用于在体内评估药物对表皮增殖和分化的影响。
