†Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
‡Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Anal Chem. 2015 Apr 7;87(7):3694-8. doi: 10.1021/acs.analchem.5b00887. Epub 2015 Mar 27.
A novel hydrophilic fluorescence temperature probe (PNDP) based on polarity-sensitive triarylboron compound (DPTB) and PNIPAM is designed and synthesized. In order to overcome the shortcomings of the single-intensity-based sensing mechanism and obtain more robust signals, ratiometric readout is achieved by designing an efficient FRET system (PNDP-NR) between DPTB and Nile Red (NR). PNDP-NR possesses some excellent features, including wide temperature range, good linear relationship, high temperature resolution, excellent reversibility, and stability. Within a sensing temperature range of 30-55 °C, the fluorescence color of PNDP-NR experiences significant change from red to green-blue. PNDP-NR is also introduced into NIH/3T3 cells to sense the temperature at the single-cell level. It gave excellent photostability and low cytotoxicity in vivo.
一种基于亲水性荧光温度探针（PNDP）的新型极性敏感三芳基硼化合物（DPTB）和 PNIPAM 被设计和合成。为了克服基于单一强度的传感机制的缺点，并获得更稳健的信号，通过设计 DPTB 和尼罗红（NR）之间有效的 FRET 系统（PNDP-NR）实现了比率读出。PNDP-NR 具有一些优异的特性，包括宽温度范围、良好的线性关系、高温度分辨率、优异的可逆性和稳定性。在 30-55°C 的传感温度范围内，PNDP-NR 的荧光颜色从红色到蓝绿色发生显著变化。PNDP-NR 还被引入 NIH/3T3 细胞中以在单细胞水平上感应温度。它在体内具有优异的光稳定性和低细胞毒性。
