Uchiyama Seiichi, Tsuji Toshikazu, Ikado Kumiko, Yoshida Aruto, Kawamoto Kyoko, Hayashi Teruyuki, Inada Noriko
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Analyst. 2015 Jul 7;140(13):4498-506. doi: 10.1039/c5an00420a.
We developed new cationic fluorescent polymeric thermometers containing both benzothiadiazole and BODIPY units as an environment-sensitive fluorophore and as a reference fluorophore, respectively. The temperature-dependent fluorescence spectra of the thermometers enabled us to perform highly sensitive and practical ratiometric temperature sensing inside living mammalian cells. Intracellular temperatures of non-adherent MOLT-4 (human acute lymphoblastic leukaemia) and adherent HEK293T (human embryonic kidney) cells could be monitored with high temperature resolutions (0.01-1.0 °C) using the new cationic fluorescent polymeric thermometer.
我们开发了新型阳离子荧光聚合物温度计，其分别包含苯并噻二唑和BODIPY单元，作为环境敏感荧光团和参比荧光团。这些温度计随温度变化的荧光光谱使我们能够在活的哺乳动物细胞内进行高灵敏度且实用的比率温度传感。使用这种新型阳离子荧光聚合物温度计，可以以高温度分辨率（0.01 - 1.0°C）监测非贴壁MOLT-4（人急性淋巴细胞白血病）细胞和贴壁HEK293T（人胚肾）细胞的细胞内温度。
