Knapinska Anna M, Tokmina-Roszyk Dorota, Amar Sabrina, Tokmina-Roszyk Michal, Mochalin Vadym N, Gogotsi Yury, Cosme Patrick, Terentis Andrew C, Fields Gregg B
Department of Chemistry and Biochemistry, Florida Atlantic University, Jupiter, FL, 33458.
Departments of Chemistry and Biology, Torrey Pines Institute for Molecular Studies, Port St. Lucie, FL, 34987.
Biopolymers. 2015 May;104(3):186-95. doi: 10.1002/bip.22636.
Nanodiamonds (NDs) have received considerable attention as potential drug delivery vehicles. NDs are small (∼5 nm diameter), can be surface modified in a controllable fashion with a variety of functional groups, and have little observed toxicity in vitro and in vivo. However, most biomedical applications of NDs utilize surface adsorption of biomolecules, as opposed to covalent attachment. Covalent modification provides reliable and reproducible ND-biomolecule ratios, and alleviates concerns over biomolecule desorption prior to delivery. The present study has outlined methods for the efficient solid-phase conjugation of ND to peptides and characterization of ND-peptide conjugates. Utilizing collagen-derived peptides, the ND was found to support or even enhance the cell adhesion and viability activities of the conjugated sequence. Thus, NDs can be incorporated into peptides and proteins in a selective manner, where the presence of the ND could potentially enhance the in vivo activities of the biomolecule it is attached to.
纳米金刚石（NDs）作为潜在的药物递送载体受到了广泛关注。NDs体积小（直径约5纳米），可以通过各种官能团以可控方式进行表面修饰，并且在体外和体内几乎没有观察到毒性。然而，NDs的大多数生物医学应用利用生物分子的表面吸附，而非共价连接。共价修饰提供了可靠且可重复的ND-生物分子比率，并消除了对递送前生物分子解吸的担忧。本研究概述了将ND高效固相缀合到肽上的方法以及ND-肽缀合物的表征。利用胶原蛋白衍生的肽，发现ND能够支持甚至增强缀合序列的细胞粘附和活力活性。因此，NDs可以以选择性方式掺入肽和蛋白质中，其中ND的存在可能会增强其所连接生物分子的体内活性。
