Jourdain A, Semba K, Fibiger H C
Department of Psychiatry, University of British Columbia, Vancouver, Canada.
Brain Res. 1989 Dec 25;505(1):55-65. doi: 10.1016/0006-8993(89)90115-7.
Using a double fluorescence retrograde labeling procedure, the present study sought to determine the degree to which basal forebrain and mesopontine tegmental neurons have axons that innervate both the reticular thalamic nucleus and the cerebral cortex. Immunofluorescence for choline acetyltransferase, somatostatin, and the calcium-binding protein parvalbumin was also performed to elucidate the neurochemical identity of basal forebrain and mesopontine tegmental inputs to the reticular thalamic nucleus. A significant portion (10-15%) of neurons in the basal forebrain and mesopontine tegmentum that were retrogradely labeled from the reticular thalamic nucleus were also found to be retrogradely labeled from the cortex. Many of these neurons stained positively for choline acetyltransferase. Of the basal forebrain neurons retrogradely labeled from the reticular thalamic nucleus, approximately 20% were found to be immunoreactive to choline acetyltransferase, whereas none was stained for somatostatin. A larger portion (up to 50%) of the basal forebrain neurons that were retrogradely labeled from the reticular thalamic nucleus were parvalbumin-immunoreactive, and some of these were also retrogradely labeled from the cortex. These results suggest that a subpopulation of cholinergic and non-cholinergic neurons in the basal forebrain and the mesopontine tegmentum may influence simultaneously the activity of neurons in the reticular thalamic nucleus and the cerebral cortex.
本研究采用双荧光逆行标记法，旨在确定基底前脑和中脑桥被盖区神经元的轴突支配丘脑网状核和大脑皮层的程度。还进行了胆碱乙酰转移酶、生长抑素和钙结合蛋白小白蛋白的免疫荧光检测，以阐明基底前脑和中脑桥被盖区向丘脑网状核输入的神经化学特性。从丘脑网状核逆行标记的基底前脑和中脑桥被盖区的神经元中，有相当一部分（10 - 15%）也被发现从皮层逆行标记。这些神经元中有许多胆碱乙酰转移酶染色呈阳性。在从丘脑网状核逆行标记的基底前脑神经元中，约20%被发现对胆碱乙酰转移酶具有免疫反应性，而生长抑素染色均为阴性。从丘脑网状核逆行标记的基底前脑神经元中，较大比例（高达50%）对小白蛋白具有免疫反应性，其中一些也从皮层逆行标记。这些结果表明，基底前脑和中脑桥被盖区的胆碱能和非胆碱能神经元亚群可能同时影响丘脑网状核和大脑皮层神经元的活动。
