Jahromi S R, Haddadi M, Shivanandappa T, Ramesh S R
Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India.
Department of Biology, Faculty of Science, University of Zabol, Zabol, Iran.
Neuroscience. 2015 May 7;293:136-50. doi: 10.1016/j.neuroscience.2015.02.048. Epub 2015 Mar 6.
Oxidative stress is believed to be a major factor for the onset of Parkinson's disease (PD). In this study, we have investigated oxidative status in transgenic Drosophila model of PD. Our results revealed elevated levels of reactive oxygen species (ROS) and lipid peroxidation (LPO) in A30P and A53T α-synuclein PD model flies compared to control. We have demonstrated for the first time the ameliorating potential of natural antioxidants characterized from the roots of Dh in A30P and A53T α-synuclein PD model flies. Feeding of transgenic flies with aqueous Dh root extract for 21 days significantly improved their climbing ability and circadian rhythm of locomotor activity which was associated with reduction in levels of ROS and LPO and enhancement in the activities of catalase (CAT) and superoxide dismutase (SOD). Dh protected against paraquat (PQ) sensitivity in α-synuclein transgenic flies and delayed the onset of PD-like symptoms which appears to be mediated by suppression of oxidative stress.
氧化应激被认为是帕金森病（PD）发病的一个主要因素。在本研究中，我们调查了PD转基因果蝇模型中的氧化状态。我们的结果显示，与对照相比，A30P和A53T α-突触核蛋白PD模型果蝇中的活性氧（ROS）水平和脂质过氧化（LPO）水平升高。我们首次证明了从Dh根中提取的天然抗氧化剂对A30P和A53T α-突触核蛋白PD模型果蝇具有改善潜力。用Dh根水提取物喂养转基因果蝇21天，显著改善了它们的攀爬能力和运动活动的昼夜节律，这与ROS和LPO水平的降低以及过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性的增强有关。Dh可保护α-突触核蛋白转基因果蝇免受百草枯（PQ）敏感性影响，并延迟PD样症状的出现，这似乎是通过抑制氧化应激介导的。
