神圣罗勒叶提取物在帕金森病转基因果蝇模型中对膳食补充的作用。
Role of Ocimum sanctum leaf extract on dietary supplementation in the transgenic Drosophila model of Parkinson's disease.
作者信息
Siddique Yasir Hasan, Faisal Mohammad, Naz Falaq, Jyoti Smita
机构信息
Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
Forest Entomology Division, Forest Research Institute, Dehradun 248006, UK, India.
出版信息
Chin J Nat Med. 2014 Oct;12(10):777-81. doi: 10.1016/S1875-5364(14)60118-7. Epub 2014 Oct 31.
AIM
To evaluate the effect of Ocimum sanctum leaf extract on the dietary supplementation in the transgenic Drosophila model of Parkinson's disease.
METHOD
The effect of Ocimum sanctum leaf extract was studied on the transgenic Drosophila model of flies expressing normal human alpha synuclein (h-αs) in the neurons. O. sanctum extract at final concentrations of 0.042 8 × 10(-4), 0.87 × 10(-4), and 1.85 × 10(-4) g·mL(-1) of diet were established and the flies were allowed to feed for 21 days. The climbing assay and lipid peroxidation were taken as parameters for the study.
RESULTS
The supplementation of O. sanctum extract showed a dose-dependent significant delay in the loss of climbing ability and reduction in oxidative stress in the brain of PD model flies.
CONCLUSION
The results of the present study showed that the O. sanctum extract is potent in reducing the PD symptoms in transgenic Drosophila model.
目的
评估神圣罗勒叶提取物对帕金森病转基因果蝇模型饮食补充的影响。
方法
研究神圣罗勒叶提取物对在神经元中表达正常人α-突触核蛋白（h-αs）的转基因果蝇模型的影响。建立了终浓度为0.042 8×10⁻⁴、0.87×10⁻⁴和1.85×10⁻⁴ g·mL⁻¹饮食的神圣罗勒提取物，并让果蝇进食21天。采用攀爬试验和脂质过氧化作为研究参数。
结果
补充神圣罗勒提取物显示出剂量依赖性地显著延迟帕金森病模型果蝇大脑攀爬能力的丧失并降低氧化应激。
结论
本研究结果表明，神圣罗勒提取物在减轻转基因果蝇模型中的帕金森病症状方面具有效力。
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