Gao Jing, Zou Zhengzhi, Gao Jing, Zhang Haifang, Lin Zhicai, Zhang Yaya, Luo Xianyang, Liu Changhua, Xie Jingdun, Cai Chengfu
Department of Head and Neck Surgery, First Affiliated Hospital of Xiamen University Xiamen, China.
MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University Guangzhou, China.
Int J Clin Exp Pathol. 2015 Jan 1;8(1):345-52. eCollection 2015.
HMGB3, an X-linked member of the high-mobility group (HMG) superfamily of HMG proteins, has been shown to affect numerous tumorigenic progression. However, the expression and the prognostic role of HMGB3 in esophageal squamous cell carcinoma (ESCC) remained unknown. In this study, we examined the HMGB3 expression in ESCC tissues and adjacent nontumorous tissues by qRT-PCR and immuohistochemistry. Statistical analyses were applied to test for prognostic and diagnostic associations. The mRNA levels of HMGB3 were found to be significantly higher in tumorous tissues than in the adjacent normal tissues. We found that the HMGB3 expression was higher in tumorous tissues than in the adjacent non-tumorous tissues by immunohistochemical analysis of paired tissue specimens (P < 0.001). Moreover, there was a significant correlation between HMGB3 expression and gender (P = 0.037), clinical stage (P = 0.038), T classification (P = 0.013) and N classification (P = 0.017). Patients with higher HMGB3 expression had shorter overall survival than those with lower HMGB3 expression. Multivariate Cox analysis indicated that HMGB3 expression is an independent prognostic factor for overall survival (HR = 0.591, 95% CI = 0.379-0.793, P = 0.039). In summary, these findings demonstrate that HMBG3 may be a potential molecular marker for predicting the prognosis of ESCC patients.
HMGB3是高迁移率族（HMG）蛋白超家族中一个与X染色体连锁的成员，已被证明会影响多种肿瘤发生进程。然而，HMGB3在食管鳞状细胞癌（ESCC）中的表达及预后作用尚不清楚。在本研究中，我们通过qRT-PCR和免疫组化检测了ESCC组织及相邻非肿瘤组织中HMGB3的表达情况。应用统计学分析来检验预后和诊断相关性。结果发现，HMGB3的mRNA水平在肿瘤组织中显著高于相邻正常组织。通过对配对组织标本进行免疫组化分析，我们发现肿瘤组织中HMGB3的表达高于相邻非肿瘤组织（P < 0.001）。此外，HMGB3表达与性别（P = 0.037）、临床分期（P = 0.038）、T分级（P = 0.013）和N分级（P = 0.017）之间存在显著相关性。HMGB3表达较高的患者总生存期短于HMGB3表达较低的患者。多因素Cox分析表明，HMGB3表达是总生存期的独立预后因素（HR = 0.591，95% CI = 0.379 - 0.793，P = 0.039）。总之，这些发现表明HMBG3可能是预测ESCC患者预后的潜在分子标志物。
