Schlupp P, Weber M, Schmidts T, Geiger K, Runkel F
Institute of Bioprocess Engineering and Pharmaceutical Technology, Technische Hochschule Mittelhessen, University of Applied Sciences, Giessen, Germany.
Results Pharma Sci. 2014 Sep 6;4:26-33. doi: 10.1016/j.rinphs.2014.09.002. eCollection 2014.
Pharmaceuticals and cosmetics for dermal application are usually tested on healthy skin, although the primary permeation barrier, the stratum corneum, is often impaired by skin diseases or small skin lesions, especially on the hands. These skin conditions can considerably influence the permeation of chemicals and drugs. Furthermore, risk assessment for example of nanoparticles should be performed under various skin conditions to reflect the true circumstances. Therefore, an alternative and reproducible method for a high throughput of skin samples with impaired skin barrier was developed and verified by skin permeation studies (25 h) of caffeine, sorbic acid and testosterone compared to healthy (untreated) and tape-stripped skin. Skin barrier disruption was controlled by TEWL measurement. Skin permeation of the three substances was increased in tape-stripped and abraded skin compared to untreated skin due to the reduced barrier integrity. Enhancement of drug uptake was highest for the most hydrophilic substance, caffeine, followed by sorbic acid and lipophilic testosterone. No significant difference in drug uptake studies was observed between the new abrasion method with an aluminum-coated sponge and the tape-stripping method. The obtained results demonstrate that this abrasion method is an alternative way to achieve a disturbed skin barrier for drug and chemical uptake studies.
用于皮肤给药的药品和化妆品通常在健康皮肤上进行测试，尽管主要的渗透屏障——角质层，经常会因皮肤疾病或小的皮肤损伤而受损，尤其是在手部。这些皮肤状况会极大地影响化学物质和药物的渗透。此外，例如纳米颗粒的风险评估应该在各种皮肤状况下进行，以反映真实情况。因此，开发了一种用于高通量处理皮肤屏障受损的皮肤样本的替代且可重复的方法，并通过咖啡因、山梨酸和睾酮在皮肤屏障受损的皮肤样本上进行25小时的皮肤渗透研究与健康（未处理）和胶带剥离皮肤进行比较来进行验证。通过经皮水分流失（TEWL）测量来控制皮肤屏障的破坏。由于屏障完整性降低，与未处理皮肤相比，胶带剥离和磨损皮肤中这三种物质的皮肤渗透增加。对于亲水性最强的物质咖啡因，药物摄取的增强最高，其次是山梨酸和亲脂性的睾酮。在使用涂铝海绵的新磨损方法和胶带剥离方法之间，在药物摄取研究中未观察到显著差异。获得的结果表明，这种磨损方法是在药物和化学物质摄取研究中实现皮肤屏障受损的一种替代方法。
