Jiang En-Ping, Li He, Yu Chun-Rong, Yu Chun-Yan, Jing Shu, Sun Hong-Xia, Wang Chun-Mei, Fan Xin-Tian, Chen Jian-Guang, Wang Sen
aCancer Institute, Guangdong Medical College, Dongguan bCollege of Pharmacy cCollege of Basic Medical, Beihua University dBeihua University Affiliated Hospital, Jilin eJOINN Laboratories, Beijing, China.
Neuroreport. 2015 Apr 15;26(6):360-6. doi: 10.1097/WNR.0000000000000354.
Increasing evidence places Schisandrin B (Sch B) at an important position in nerve protection, indicating that Sch B might play a positive role in the therapy of neurodegenerative diseases. However, there is little information on it. Our studies showed that pretreatment with Sch B could reduce lactate dehydrogenase, malondialdehyde, and reactive oxygen species release and significantly increase the cell viability and the superoxide dismutase level. Sch B (10 μM) markedly inhibited cell apoptosis, whereas LY294002 (20 μM), a phosphatidylinositol-3 kinase inhibitor, blocked the antiapoptotic effect. More importantly, Sch B (10 μM) increased the phosphoprotein kinase B/protein kinase B (Akt) and B-cell lymphoma-2/Bcl-2 associated X protein ratios on preincubation with cells for 2 h, which was then inhibited by LY294002 (20 μM). Results indicate that Sch B can protect PC12 cells from apoptosis by activating the phosphatidylinositol-3 kinase/Akt signaling pathway and may emerge as a potential drug for neurodegenerative diseases.
越来越多的证据表明五味子乙素（Sch B）在神经保护中占据重要地位，这表明Sch B可能在神经退行性疾病的治疗中发挥积极作用。然而，关于它的信息却很少。我们的研究表明，Sch B预处理可降低乳酸脱氢酶、丙二醛和活性氧的释放，并显著提高细胞活力和超氧化物歧化酶水平。Sch B（10 μM）显著抑制细胞凋亡，而磷脂酰肌醇-3激酶抑制剂LY294002（20 μM）可阻断其抗凋亡作用。更重要的是，Sch B（10 μM）在与细胞预孵育2小时后可提高磷酸化蛋白激酶B/蛋白激酶B（Akt）和B细胞淋巴瘤-2/Bcl-2相关X蛋白的比例，随后被LY294002（20 μM）抑制。结果表明，Sch B可通过激活磷脂酰肌醇-3激酶/Akt信号通路保护PC12细胞免受凋亡，可能成为治疗神经退行性疾病的潜在药物。
