植物生长物质对蓖麻（Ricinus communis L.）含油量和脂肪酸组成的影响：体外研究。
The effects of plant growth substances on the oil content and fatty acid composition of Ricinus communis L.: an in vitro study.
机构信息
Department of Bioengineering, Faculty of Engineering, Adana Alpaslan Turkes Science and Technology University, Adana, Turkey.
出版信息
Mol Biol Rep. 2022 Jun;49(6):5241-5249. doi: 10.1007/s11033-021-06686-2. Epub 2021 Sep 1.
BACKGROUND
Ricinus communis L. (castor bean) is valued for its oil and the performance of oil is closely related to its fatty acid composition. Thus, producing oil in vitro with favored fatty acid profiles is a promising research area and may also offer industrial opportunities.
MATERIAL AND METHOD
In line with this, the total amount of oil and the fatty acid composition of the samples, which were endosperm and calli obtained by treatment of various doses of plant growth regulators were determined.
RESULTS
Results showed that the type and amount of the plant growth regulator used in the media affect the fatty acid composition. In detail, the biggest change was shown by Indole-3-Acetic Acid (IAA), in general, using the plant growth regulators at 5 mg L, instead of 20 mg L, was found to have induced larger differentiations. The effect of a natural plant growth regulator (IAA) on fatty acid profiles was bigger than the synthetic ones (NAA, 1-Naphthaleneacetic acid, and 2,4 D, 2,4-Dichlorophenoxyacetic acid). The media containing 5 mg L of NAA, 20 mg L of NAA, 20 mg L of 2,4 D, or 5 mg L of 2,4 D gave similar results.
背景
蓖麻（Ricinus communis L.）因其油而受到重视，油的性能与其脂肪酸组成密切相关。因此，体外生产具有理想脂肪酸谱的油是一个很有前途的研究领域，也可能提供工业机会。
材料与方法
为此，测定了用不同剂量植物生长调节剂处理得到的胚乳和愈伤组织的总油量和脂肪酸组成。
结果
结果表明，培养基中使用的植物生长调节剂的类型和数量影响脂肪酸组成。具体而言，吲哚乙酸（IAA）的变化最大，一般来说，使用 5mg/L 而不是 20mg/L 的植物生长调节剂，被发现能诱导更大的分化。天然植物生长调节剂（IAA）对脂肪酸谱的影响大于合成植物生长调节剂（NAA、1-萘乙酸、2,4-D 和 2,4-二氯苯氧乙酸）。含有 5mg/L NAA、20mg/L NAA、20mg/L 2,4-D 或 5mg/L 2,4-D 的培养基得出了类似的结果。
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