Amaeze Nnamdi H, Adeyemi Rosemary O, Adebesin Adeshina O
Ecotoxicology and Conservation Unit, Ecotoxicology Laboratory, Department of Zoology, University of Lagos, Akoka-Yaba, Lagos, Nigeria,
Environ Monit Assess. 2015 Apr;187(4):172. doi: 10.1007/s10661-015-4390-0. Epub 2015 Mar 11.
This study assessed the level of oxidative stress and heat shock proteins (Hsp) induced in gills and liver as well as the histopathological effects in the gills of African catfish, Clarias gariepinus, exposed to runoff water from the Third Mainland Bridge, the busiest bridge in the City of Lagos, Nigeria. The runoff was sampled off the bridge drainage, and the assessment of its physicochemical characteristics in both dry and rainy season indicated a high level of chemical and biological oxygen demand, oil and grease as well as manganese. After a preliminary acute toxicity evaluation, the juveniles of C. gariepinus were exposed to 5, 10, 15, 25, 50 and 100% runoff samples for 30 days. The level of the lipid peroxidation product, malondialdehyde, was significantly different from the control and increased serially with higher concentrations of effluent exposure. There was also an inhibition in the activities of enzymes, superoxide dismutase and catalase as well as significantly lower levels of reduced glutathione after 30 days. Heat shock proteins induced in the catfishes over this period included proteases, sHsps, Hsp 40s, Hsp 60s (for all the fishes-control and exposed) and Hsp 70s (in the exposed fishes only). Histopathological assessments of the gills indicated that the major effects of the runoff exposures were mild to severe thickening and fusion of the gill lamellae, stunting of lamellae, erosion of outer epithelium, epithelial lifting, hyperplasia, oedema and aneurism. The implications of the findings in the Lagos lagoon fishes were discussed.
本研究评估了暴露于尼日利亚拉各斯市最繁忙的第三大陆桥径流水中的非洲鲶鱼（Clarias gariepinus）鳃和肝脏中诱导产生的氧化应激水平和热休克蛋白（Hsp），以及鳃的组织病理学效应。径流取自桥排水口，对其在旱季和雨季的理化特性评估表明，化学需氧量、生化需氧量、油脂以及锰含量很高。经过初步急性毒性评估后，将非洲鲶鱼幼鱼暴露于5%、10%、15%、25%、50%和100%的径流样本中30天。脂质过氧化产物丙二醛的水平与对照组有显著差异，并随着废水暴露浓度的升高而依次增加。30天后，超氧化物歧化酶和过氧化氢酶的活性也受到抑制，还原型谷胱甘肽水平显著降低。在此期间，鲶鱼体内诱导产生的热休克蛋白包括蛋白酶、小分子热休克蛋白、热休克蛋白40、热休克蛋白60（所有鱼类——对照组和暴露组）以及热休克蛋白70（仅在暴露组鱼类中）。鳃的组织病理学评估表明，径流暴露的主要影响是鳃小片轻度至重度增厚和融合、小片发育不良、外上皮侵蚀、上皮隆起、增生、水肿和动脉瘤。讨论了这些研究结果对拉各斯泻湖鱼类的影响。
