Song Liyan, Wang Yangqing, Tang Wei, Lei Yu
Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, Beibei, Chongqing, 400714, China,
Appl Microbiol Biotechnol. 2015 Jul;99(14):6125-37. doi: 10.1007/s00253-015-6493-5. Epub 2015 Mar 12.
Despite the pivotal role of archaea in methane production in landfills, the identity, ecology, and functional diversity of these microorganisms and their link to environmental factors remain largely unknown. We collected 11 landfill leachate samples from six geographically distinct landfills of different ages in China and analyzed the archaeal community by bar-coded 454 pyrosequencing. We retrieved 121,797 sequences from a total of 167,583 sequences (average length of 464 bp). The archaeal community was geographically structured, and nonabundant taxa primarily contributed to the observed dissimilarities. Canonical correlation analysis (CCA) suggested that the total phosphorous (TP), nitrate, and conductivity are important drivers for shaping the archaeal community. The hydrogenotrophic methanogens Methanomicrobiales and Methanobacteriales greatly dominated 9 of 11 samples, ranging from 83.7 to 99.5 %. These methanogens also dominated the remaining two samples, accounting for 70.3 and 58.8 %, respectively. Interestingly, for all of the studied Chinese landfills, 16S rRNA analysis indicated the predominance of hydrogenotrophic methanogens.
尽管古菌在垃圾填埋场甲烷产生中起着关键作用，但这些微生物的身份、生态、功能多样性以及它们与环境因素的联系在很大程度上仍不清楚。我们从中国六个不同地理位置、不同年代的垃圾填埋场收集了11份垃圾渗滤液样本，并通过条形码454焦磷酸测序分析了古菌群落。我们从总共167,583条序列（平均长度464 bp）中检索到121,797条序列。古菌群落具有地理结构，非优势类群是观察到的差异的主要贡献者。典范对应分析（CCA）表明，总磷（TP）、硝酸盐和电导率是塑造古菌群落的重要驱动因素。氢营养型产甲烷菌甲烷微菌目和甲烷杆菌目在11个样本中的9个中占主导地位，范围从83.7%到99.5%。这些产甲烷菌在其余两个样本中也占主导地位，分别占70.3%和58.8%。有趣的是，对于所有研究的中国垃圾填埋场，16S rRNA分析表明氢营养型产甲烷菌占优势。
