通过线粒体细胞色素C氧化酶亚基1测序对中国甘肃省多头带绦虫分离株进行分子特征分析
Molecular characterization of Taenia multiceps isolates from Gansu Province, China by sequencing of mitochondrial cytochrome C oxidase subunit 1.
作者信息
Li Wen Hui, Jia Wan Zhong, Qu Zi Gang, Xie Zhi Zhou, Luo Jian Xun, Yin Hong, Sun Xiao Lin, Blaga Radu, Fu Bao Quan
机构信息
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
出版信息
Korean J Parasitol. 2013 Apr;51(2):197-201. doi: 10.3347/kjp.2013.51.2.197. Epub 2013 Apr 25.
A total of 16 Taenia multiceps isolates collected from naturally infected sheep or goats in Gansu Province, China were characterized by sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The complete cox1 gene was amplified for individual T. multiceps isolates by PCR, ligated to pMD18T vector, and sequenced. Sequence analysis indicated that out of 16 T. multiceps isolates 10 unique cox1 gene sequences of 1,623 bp were obtained with sequence variation of 0.12-0.68%. The results showed that the cox1 gene sequences were highly conserved among the examined T. multiceps isolates. However, they were quite different from those of the other Taenia species. Phylogenetic analysis based on complete cox1 gene sequences revealed that T. multiceps isolates were composed of 3 genotypes and distinguished from the other Taenia species.
对从中国甘肃省自然感染的绵羊或山羊中采集的16株多头绦虫分离株，通过线粒体细胞色素c氧化酶亚基1（cox1）基因序列进行特征分析。通过PCR扩增单个多头绦虫分离株的完整cox1基因，连接到pMD18T载体上并进行测序。序列分析表明，16株多头绦虫分离株中获得了10个独特的1623 bp的cox1基因序列，序列变异为0.12 - 0.68%。结果表明，cox1基因序列在检测的多头绦虫分离株中高度保守。然而，它们与其他绦虫种类的序列有很大不同。基于完整cox1基因序列的系统发育分析表明，多头绦虫分离株由3个基因型组成，并与其他绦虫种类区分开来。
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