Department of Pediatric Surgery, Derince Training and Research Hospital, Kocaeli, Turkey.
Department of Emergency Medicine, Abant İzzet Baysal University Faculty of Medicine, Bolu, Turkey.
Balkan Med J. 2015 Jan;32(1):96-100. doi: 10.5152/balkanmedj.2015.15459. Epub 2015 Jan 1.
Oesophageal perforation is a life-threatening pathology that is generally treated conservatively; however, surgical procedures are frequently performed. A topical haemostatic agent, Ankaferd Blood Stopper (ABS), also has beneficial wound-healing effects.
This study aimed to determine the effects of ABS following experimental oesophageal perforations.
Animal experiment.
The experimental rats were classified into 6 groups (with 7 rats in each group). Pairs of groups (primary repair alone and primary repair + ABS) were terminated in the 1(st), 2(nd), and 3(rd) weeks following injury. The oesophageal perforations, which were 8-10 mm in length, were created using a nasogastric tube. The perforation sites were repaired with a 6-0 polyglactine thread in the primary repair groups. Additionally, ABS was sprayed over the perforation site in the treatment groups. Each oesophagus was evaluated histopathologically.
There were fewer microabscesses and areas of necrosis in the ABS groups compared with the primary repair groups. The histopathological evaluation revealed that the ABS groups had less inflammation and more re-epithelisation compared to the primary repair groups (p=0.002 and p=0.003, respectively). Fibrosis in the ABS groups was moderate in the 2(nd) week and mild in the 3(rd) week. Comparing the groups with respect to the time intervals, only the 1(st) week groups showed a significant difference in terms of re-epithelialisation (p=0.044).
Topical ABS application on the repaired experimental oesophageal perforation regions led to positive wound-healing effects compared with the rats that were administered the primary repair alone.
食管穿孔是一种危及生命的疾病，通常采用保守治疗；然而，手术治疗也经常进行。一种局部止血剂，安可福止血粉（ABS），也具有有益的伤口愈合作用。
本研究旨在确定 ABS 对实验性食管穿孔的影响。
动物实验。
将实验大鼠分为 6 组（每组 7 只）。每组中的一对（单纯一期修复和一期修复+ABS）在损伤后第 1、2 和 3 周终止。使用鼻胃管在食管上造成 8-10mm 长的穿孔。在一期修复组中，用 6-0 聚甘醇缝线修复穿孔部位。在治疗组中，在穿孔部位喷洒 ABS。每个食管都进行组织病理学评估。
与单纯一期修复组相比，ABS 组的微脓肿和坏死面积较少。组织病理学评估显示，与单纯一期修复组相比，ABS 组的炎症较轻，再上皮化程度较高（p=0.002 和 p=0.003）。ABS 组在第 2 周的纤维化程度为中度，在第 3 周为轻度。比较各组的时间间隔，只有第 1 周组的再上皮化有显著差异（p=0.044）。
与单纯一期修复组相比，在修复后的实验性食管穿孔部位局部应用 ABS 可产生积极的伤口愈合作用。
