Laboratório de Enzimologia, Departamento de Biologia Celular, Universidade de Brasília , Brasília, DF , Brasil.
Braz J Microbiol. 2012 Apr;43(2):498-505. doi: 10.1590/S1517-83822012000200010. Epub 2012 Jun 1.
Holocellulose structures from agro-industrial residues rely on main and side chain attacking enzymes with different specificities for complete hydrolysis. Combinations of crude enzymatic extracts from different fungal species, including Aspergillus terreus, Aspergillus oryzae, Aspergillus niger and Trichoderma longibrachiatum, were applied to sugar cane bagasse, banana stem and dirty cotton residue to investigate the hydrolysis of holocellulose structures. A. terreus and A. oryzae were the best producers of FPase and xylanase activities. A combination of A. terreus and A. oryzae extracts in a 50% proportion provided optimal hydrolysis of dirty cotton residue and banana stem. For the hydrolysis of sugar cane bagasse, the best results were obtained with samples only containing A. terreus crude extract.
依赖于具有不同特异性的主链和侧链攻击酶的农业工业残留物全纤维素结构，才能实现完全水解。从不同真菌物种（包括土曲霉、米曲霉、黑曲霉和长枝木霉）的粗酶提取物组合，应用于甘蔗渣、香蕉茎和脏棉废料，以研究全纤维素结构的水解。A. terreus 和 A. oryzae 是 FPase 和木聚糖酶活性的最佳产生菌。A. terreus 和 A. oryzae 提取物以 50%的比例组合，为脏棉废料和香蕉茎的最佳水解提供了条件。对于甘蔗渣的水解，仅含有 A. terreus 粗提物的样品获得了最佳效果。
