Okyay Tugba Onal, Rodrigues Debora F
Department of Civil and Environmental Engineering, University of Houston, Houston, TX 77204-4003, USA.
Department of Civil and Environmental Engineering, University of Houston, Houston, TX 77204-4003, USA
FEMS Microbiol Ecol. 2015 Mar;91(3). doi: 10.1093/femsec/fiv017. Epub 2015 Feb 11.
In this study, CO2 sequestration was investigated through the microbially-induced calcium carbonate precipitation (MICP) process with isolates obtained from a cave called 'Cave Without A Name' (Boerne, TX, USA) and the Pamukkale travertines (Denizli, Turkey). The majority of the bacterial isolates obtained from these habitats belonged to the genera Sporosarcina, Brevundimonas, Sphingobacterium and Acinetobacter. The isolates were investigated for their capability to precipitate calcium carbonate and sequester CO2. Biotic and abiotic effects of CO2 sequestration during MICP were also investigated. In the biotic effect, we observed that the rate and concentration of CO2 sequestered was dependent on the species or strains. The main abiotic factors affecting CO2 sequestration during MICP were the pH and medium components. The increase in pH led to enhanced CO2 sequestration by the growth medium. The growth medium components, on the other hand, were shown to affect both the urease activity and CO2 sequestration. Through the Plackett-Burman experimental design, the most important growth medium component involved in CO2 sequestration was determined to be urea. The optimized medium composition by the Plackett-Burman design for each isolate led to a statistically significant increase, of up to 148.9%, in CO2 uptake through calcification mechanisms.
在本研究中，通过微生物诱导碳酸钙沉淀（MICP）过程，利用从名为“无名洞穴”（美国得克萨斯州博尔尼）和土耳其代尼兹利的棉花堡钙华获取的分离菌株，对二氧化碳封存进行了研究。从这些栖息地获得的大多数细菌分离株属于芽孢八叠球菌属、短波单胞菌属、鞘氨醇杆菌属和不动杆菌属。研究了这些分离菌株沉淀碳酸钙和封存二氧化碳的能力。还研究了MICP过程中二氧化碳封存的生物和非生物效应。在生物效应方面，我们观察到封存的二氧化碳速率和浓度取决于物种或菌株。影响MICP过程中二氧化碳封存的主要非生物因素是pH值和培养基成分。pH值的升高导致生长培养基对二氧化碳的封存增强。另一方面，生长培养基成分显示出对脲酶活性和二氧化碳封存都有影响。通过Plackett-Burman实验设计，确定参与二氧化碳封存的最重要生长培养基成分是尿素。通过Plackett-Burman设计对每种分离菌株进行优化的培养基组成，导致通过钙化机制吸收的二氧化碳在统计上显著增加，增幅高达148.9%。
