Chanchaem Prangwalai, Poovorawan Yong, Payungporn Sunchai
J Med Assoc Thai. 2015 Jan;98 Suppl 1:S29-35.
The WU polyomavirus (WUPyV) is a small DNA virus (family Polyomaviridae) that contains a circular double-stranded DNA genome, approximately 5 kb in length. WUPyV was first discovered in the respiratory tract of children with acute respiratory symptoms.
This study focuses on the complete genome characterization and phylogenetic analysis of WUPyV obtained from Thai patients with respiratory diseases in 2013.
DNA was extracted from nasopharyngeal (NP) suction specimens (n = 614) from patients with respiratory tract infections. WUPyV was detected by using semi-nested PCR and then characterized by whole genome sequencing. The nucleotides and deduced amino acid sequences were analyzed by multiple sequences alignment and phylogenetic tree.
Analysis revealed that 0.16% (1/614) of the sample was positive for WUPyV. Phylogenetic trees demonstrated that WUPyV(isolate CU_Chonburi 3) was closely related to previously described WUPyV. Moreover, whole genome sequences alignment of WUPyV showed several nucleotide variations within non-coding regions and amino acid changes in VP1 (position S347T); VP2 (positions L40V, G120R, Y121I, P123R, G127S, L137F, Q287R, and A327V); LTAg (positions Q357P, V369E, E377K, D378V, A381T, R382E, R383G, and D389G); and, STAg (positions R139S, K141E, R148K, and W153C).
Nucleotide variations within non-coding regions and critical amino acid substitutions in viral proteins may affect the rate of viral replication and viral adaptation; factors that may be linked to the susceptibility and severity of viral infection. Data obtained from this study may be useful in better understanding the genetic characterization and mutation patterns of WUPyV.
WU多瘤病毒（WUPyV）是一种小型DNA病毒（多瘤病毒科），其基因组为环状双链DNA，长度约为5 kb。WUPyV最初在患有急性呼吸道症状的儿童呼吸道中被发现。
本研究聚焦于2013年从泰国呼吸道疾病患者中获得的WUPyV的全基因组特征分析和系统发育分析。
从呼吸道感染患者的鼻咽抽吸标本（n = 614）中提取DNA。使用半巢式PCR检测WUPyV，然后通过全基因组测序进行特征分析。通过多序列比对和系统发育树分析核苷酸和推导的氨基酸序列。
分析显示，0.16%（1/614）的样本WUPyV呈阳性。系统发育树表明，WUPyV（分离株CU_Chonburi 3）与先前描述的WUPyV密切相关。此外，WUPyV的全基因组序列比对显示，非编码区内有多个核苷酸变异，VP1（位置S347T）、VP2（位置L40V、G120R、Y121I、P123R、G127S、L137F、Q287R和A327V）、大T抗原（LTAg，位置Q357P、V369E、E377K、D378V、A381T、R382E、R383G和D389G）以及小T抗原（STAg，位置R139S、K141E、R148K和W153C）有氨基酸变化。
非编码区内的核苷酸变异和病毒蛋白中的关键氨基酸替代可能影响病毒复制率和病毒适应性；这些因素可能与病毒感染的易感性和严重程度有关。本研究获得的数据可能有助于更好地理解WUPyV的遗传特征和突变模式。
