研究卡罗林斯卡学院和华盛顿大学多瘤病毒在扁桃体、腺样体、咽喉拭子和中耳液样本中的情况。
Study of Karolinska Institutet and Washington University polyomaviruses in tonsil, adenoid, throat swab and middle ear fluid samples.
机构信息
Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98., H-4032 Debrecen, Hungary.
Military Medical Centre, Hungarian Defence Forces, Róbert Károly körút 6, H-1134 Budapest, Hungary.
出版信息
Future Microbiol. 2018 Dec;13:1719-1730. doi: 10.2217/fmb-2018-0280. Epub 2018 Nov 28.
AIM
To study prevalence of Karolinska Institutet (KI) and Washington University (WU) polyomavirus (PyV) in 100 tonsils, 100 adenoids, 146 throat swab and 15 middle ear fluid samples collected from 146 patients (120 children and 26 adults), to analyze the sequence of noncoding control region (NCCR) and complete WUPyV genomes.
MATERIALS & METHODS: Viruses were detected by quantitative real-time PCR. The NCCRs and WUPyV genomes were sequenced and analyzed.
RESULTS
The frequency of WUPyV and KIPyV DNA was 27 and 11% in adenoids, 4 and 3% in tonsils, 4.1 and 1.4% in throat swab samples, respectively. The WUPyV DNA was detected in one middle ear fluid sample as well. The WUPyV NCCRs showed mutations which may alter the putative transcription factor binding sites. Phylogenetic analysis revealed three clades of WUPyV.
CONCLUSION
Tonsils and adenoids might be site of virus replication and/or persistence, and WUPyV may invade into the middle ear.
目的
研究卡罗林斯卡研究所（KI）和华盛顿大学（WU）多瘤病毒（PyV）在 100 个扁桃体、100 个腺样体、146 个咽拭子和 15 个中耳液样本中的流行情况，这些样本均来自 146 名患者（120 名儿童和 26 名成人），并分析非编码控制区（NCCR）和完整的 WUPyV 基因组序列。
材料与方法
通过实时定量 PCR 检测病毒。对 NCCR 和 WUPyV 基因组进行测序和分析。
结果
在腺样体中，WUPyV 和 KIPyV DNA 的频率分别为 27%和 11%，在扁桃体中分别为 4%和 3%，在咽拭子样本中分别为 4.1%和 1.4%。在一个中耳液样本中也检测到了 WUPyV DNA。WUPyV NCCR 显示出可能改变潜在转录因子结合位点的突变。系统进化分析显示 WUPyV 分为三个分支。
结论
扁桃体和腺样体可能是病毒复制和/或持续存在的部位，WUPyV 可能侵入中耳。
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