Caramoy Albert, Kearns Victoria R, Chan Yau Kei, Hagedorn Nadine, Poole Robert J, Wong David, Fauser Sascha, Kugler Wilfried, Kirchhof Bernd, Williams Rachel L
Department of Ophthalmology, University of Cologne, Cologne, Germany.
Department of Eye and Vision Science, University of Liverpool, Liverpool, United Kingdom
J Biomater Appl. 2015 Aug;30(2):212-20. doi: 10.1177/0885328215575623. Epub 2015 Mar 11.
Developing new blends of heavier-than-water silicone oil tamponade agents containing high molecular weight polydimethylsiloxane polymer for use in vitreoretinal surgery.
The viscoelastic properties of heavier-than-water silicone oil blends (30.5% F6H8 + 69.5% polydimethylsiloxane) containing high molecular weight polymer additive at increasing concentrations were measured using a controlled-stress rheometer (TA Instruments Rheolyst AR 1000 N). Emulsification of the blends was induced using a sonication device and a pluronic surfactant as a strong emulsifier. The percentage emulsion area was photographed and measured using ImageJ software. In a second in vitro emulsification assessment, silicone oil blends were dispersed using a high shear homogenizer and the oil-in-water droplets were counted using a coulter counter particle analyser.
The addition of the high molecular weight polymer increased shear viscosity and viscoelasticity of the oil blends, which were measureable and to some extent predictable. The in vitro emulsification models produced contradictory results. This demonstrates the difficulty of designing and using in vitro models to evaluate the emulsification tendency of tamponade agents in vivo.
Addition of a high molecular weight polymer to heavy silicone oil can increase the viscoelasticity. These findings might contribute to the development of emulsification resistant heavy silicone oils.
研发用于玻璃体视网膜手术的新型比水重的硅油填塞剂混合物，其含有高分子量聚二甲基硅氧烷聚合物。
使用控制应力流变仪（TA仪器公司的Rheolyst AR 1000 N）测量含有不同浓度高分子量聚合物添加剂的比水重的硅油混合物（30.5% F6H8 + 69.5%聚二甲基硅氧烷）的粘弹性。使用超声设备和作为强乳化剂的普朗尼克表面活性剂诱导混合物乳化。使用ImageJ软件拍摄并测量乳液面积百分比。在第二项体外乳化评估中，使用高剪切均质器分散硅油混合物，并使用库尔特计数器颗粒分析仪对水包油液滴进行计数。
高分子量聚合物的添加增加了油混合物的剪切粘度和粘弹性，这些是可测量的且在一定程度上是可预测的。体外乳化模型产生了相互矛盾的结果。这表明设计和使用体外模型来评估体内填塞剂的乳化倾向存在困难。
向重硅油中添加高分子量聚合物可增加粘弹性。这些发现可能有助于开发抗乳化重硅油。
