Salazar Marcio, Hernandes Luzmarina, Ramos Adilson Luiz, Salazar Brunamélia de Oliveira, Micheletti Kelly Regina, Paranhos Luiz Renato, de Mendonça Marcos Rogério, Cuoghi Osmar Aparecido
Department of Odontology, Universidade Federal de Sergipe, Aracaju, Sergipe, Brazil.
Department of Morphological Sciences, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
Arch Oral Biol. 2015 May;60(5):776-81. doi: 10.1016/j.archoralbio.2015.02.003. Epub 2015 Feb 20.
The aim of the present study was to evaluate the effect of two different dosages of alendronate on induced orthodontic movement in an experimental model involving rats with osteoporosis following ovariectomy.
Female Wistar rats (Rattus norvegicus) eight weeks of age were divided into four groups (n=12/group): ovariectomized (OVX group); ovariectomized and treated with alendronate sodium at 1mg/kg (Group OVX+ALN1); ovariectomized and treated with alendronate sodium at 2mg/kg (Group OVX+ALN2); and sham operated (control). Three months after ovariectomy, the maxillary right first molar was submitted to movement for five and seven days. After the death of the animals, the maxilla were removed and processed for microscopic evaluation. The maxillary left first molar (without movement) was used for comparison purposes in all groups. The samples were processed for the quantification of alveolar bone and tooth movement.
Intragroup comparisons showed significant movement after five and seven days (p<0.05) for all groups. Comparison among groups revealed greater tooth movement in the OVX group (p<0.05), on day 7.
Both alendronate sodium doses similarly decreased tooth movement in ovariectomized rats (p>0.05). Movement in ovariectomized+alendronate groups were also smaller than non-ovariectomized rats, however without statistical difference.
本研究旨在评估两种不同剂量的阿仑膦酸盐对去卵巢骨质疏松大鼠实验模型中诱导正畸移动的影响。
将8周龄雌性Wistar大鼠（褐家鼠）分为四组（每组n = 12）：去卵巢组（OVX组）；去卵巢并用1mg/kg阿仑膦酸钠治疗组（OVX + ALN1组）；去卵巢并用2mg/kg阿仑膦酸钠治疗组（OVX + ALN2组）；假手术组（对照组）。去卵巢3个月后，上颌右侧第一磨牙进行5天和7天的移动。动物死亡后，取出上颌骨并进行处理以进行显微镜评估。所有组均以上颌左侧第一磨牙（未移动）作为对照。对样本进行处理以量化牙槽骨和牙齿移动情况。
组内比较显示，所有组在5天和7天后均有明显移动（p < 0.05）。组间比较显示，在第7天，OVX组牙齿移动更大（p < 0.05）。
两种剂量的阿仑膦酸钠均同样降低了去卵巢大鼠的牙齿移动（p > 0.05）。去卵巢 + 阿仑膦酸盐组的移动也小于未去卵巢大鼠，但无统计学差异。
