CD2在小鼠B细胞谱系中的表达。

CD2 expression in murine B cell lineage.

作者信息

Yagita H, Nakamura T, Asakawa J, Matsuda H, Tansyo S, Iigo Y, Okumura K

机构信息

Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.

出版信息

Int Immunol. 1989;1(1):94-8. doi: 10.1093/intimm/1.1.94.

DOI:10.1093/intimm/1.1.94

PMID:2577288

Abstract

CD2 expression in murine B cell lineage was examined by flow cytometric and immunoprecipitation studies with anti-murine CD2 mAb and by Northern blot analysis. Cell surface expression of CD2 was demonstrated on all peripheral B cells and cell lines of B lineage. The murine CD2 transcript of 1.3 kb was detected in these B cells. An identical glycoprotein of 55-67 KD was precipitated from the lysates of surface radioiodinated thymocytes, splenic T and B cells, T and B lymphomas, RL male 1 and BCL-1, with anti-murine CD2 mAb. The majority of bone marrow B cells and a half of pre-B cells were found to be positive for CD2 expression. These results indicate that murine CD2 is expressed on B cell lineage at certain differentiation stages.

摘要

通过使用抗小鼠CD2单克隆抗体的流式细胞术和免疫沉淀研究以及Northern印迹分析，检测了小鼠B细胞谱系中的CD2表达。在所有外周B细胞和B谱系细胞系中均证实了CD2的细胞表面表达。在这些B细胞中检测到1.3 kb的小鼠CD2转录本。用抗小鼠CD2单克隆抗体从表面放射性碘化胸腺细胞、脾T和B细胞、T和B淋巴瘤、RL male 1和BCL-1的裂解物中沉淀出相同的55-67 KD糖蛋白。发现大多数骨髓B细胞和一半的前B细胞CD2表达呈阳性。这些结果表明，小鼠CD2在B细胞谱系的某些分化阶段表达。